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The present review paper provides us an overview on various key concepts of FRET dyes usage using layer by layer methodology. The particle based FRET systems have the advantage as homogeneous assays without the requirement of particle separation from the analyzing solution. It is known that system based on QD’s which is more of prepared for nucleic acid detection and have better substrates and have clear immobilization and Donor for FRET. There are other required surfaces which have better modifications of thick layer and create better restrictions FRET efficiency. It is also known as the polycation layers that have better charges using the same concern. It is known that various multi-layer poly electrolyte thin film and have normal referred to create effective electrostatic forces in a charged using better LBL film assembly using nucleic acids and other organic molecules.