ISSN: 2320-0189

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QTL Mapping and Gene Cloning of Durable Resistance to Blast in Rice

Kazutoshi Okuno*

Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan

*Corresponding Author:
Kazutoshi Okuno
Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572 Japan
E-mail: okuno.kazutoshi2423@gmail.com

Received date: 12/10/2015 Accepted date: 14/12/2015 Published date: 16/12/2015

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Abstract

Rice blast, caused by Pyricularia grisea Sacc., is a destructive disease of rice in most rice growing areas around the world. Breeding efforts have been made to introduce genes for blast resistance into desirable genetic background. Resistance to rice blast is categorized two types, complete (true) and incomplete (field resistance). Complete resistance is a hypersensitive reaction, often a complete form of resistance, and is characterized by a resistant infection type. More than 20 loci for complete blast resistance have been identified.

Introduction

Rice blast, caused by Pyricularia grisea Sacc., is a destructive disease of rice in most rice growing areas around the world. Breeding efforts have been made to introduce genes for blast resistance into desirable genetic background. Resistance to rice blast is categorized two types, complete (true) and incomplete (field resistance). Complete resistance is a hypersensitive reaction, often a complete form of resistance, and is characterized by a resistant infection type. More than 20 loci for complete blast resistance have been identified. Although cultivars with complete resistance were developed, the resistance has rapidly been broken down by compatible races of the pathogen. Field resistance, incomplete and usually polygenic, is a susceptible infection type that allows effective control of a parasite under natural field conditions and may be durable when exposed to new races of blast [1].

Mapping of QTL for Field Resistance to Rice Blast

QTL for field resistance to rice blast was analyzed using the progeny derived from the cross between Japanese upland and paddy rice cultivars. The frequency distribution of field resistance was continuous and the score ranged from 1 to 10. Owarihatamochi (resistant, upland) and Nipponbare (moderately susceptible, paddy), used as parents differed significantly in the blast resistance scores which were 2.8 and 8.0. QTL analysis was carried out in F4 progeny lines from the cross between Nipponbare and Owarihatamochi using a total of 118 RFLP and SSR markers. The analysis with MAPMAKER/QTL revealed four QTLs located on three chromosomes with LOD scores higher than 2.0. Two QTLs were detected on chromosome 4 and one QTL were detected on chromosomes 9 and 12. The phenotypic variation explained by each QTL ranged from 7.9 to 45.7% and four QTLs explained 66.3% of the total phenotypic variation. Backcrossed progeny lines were bred to transfer the QTL with largest effect on the resistance using the susceptible cultivar Aichiasahi as a recurrent parent. Among 82 F3 lines derived from the backcross, resistance segregated in the expected ratio of resistant 1: heterozygous 2: susceptible 1. The average score for blast resistance measured in the field was 4.2 ± 0.67, 7.5 ± 0.1 and 8.2 ± 0.66, for resistant, heterozygous and susceptible groups. The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G371 at a distance of 5.0 cm and 8.8 cm, respectively [2].

Positional Cloning and Function of pi21

To identify the molecular basis of pi21-mediated resistance and to settle the long-term debate about the cause of associated undesirable characteristics, we undertook map-based cloning of pi21, a recessive allele conferring field resistance to rice blast. By means of high-resolution mapping, we delimited the pi21 locus to a 1705-base pair (bp) region containing a single gene. The deduced amino acid sequence forms a protein containing a heavy metal transport/detoxification protein domain in the N-terminal region, implying that metal transport by pi21 might be associated with defense. Comparing the 1705 bp region between susceptible and resistant cultivars, seven nucleotide polymorphisms were identified, Two of them were located in the open reading frame associated with the phenotype. The resistant pi21 allele had deletions of 21 and 48 bp from one susceptible pi21 allele and of 12 and 48 bp from the other allele in the proline-rich region. A 4.7kb XbaI fragment containing susceptible pi21 allele or resistant pi21 allele was inserted into the multiple cloning site of binary vector pPZP2H-lac (S5). The construct for pi21 allele was prepared using Nipponbare (a reference cultivar of Rice Genome Project in Japan) PAC clone. pPZ-Npi21 was introduced into the resistant line carrying pi21 allele and pPZ-Opi21 was introduced into the susceptible cultivar carrying pi21 allele by Agrobacterium-mediated transformation. T1 plants containing pPZ-Opi21 did not confer resistance, whereas T1 plants containing pPZ-Npi21 increased susceptibility to blast. These results suggest that the susceptible allele negatively regulates resistance and that the resistant allele carries a loss-of-function mutation. Increased resistance by suppression of susceptible pi21 expression be means of RNAi was confirmed, providing evidence that a gene encoding a protein with a heavy metal transport/detoxification protein domain has a role in plant defense. The possible defense mechanism of pi21 is the protection from invasion of hyphae from penetrated cells into adjacent cells after inoculation of rice blast (Figure 1).

botanical-sciences-Possible-defense-mechanism

Figure 1: Possible defense mechanism of pi21 allele.

Discussion

Incompleteness, in addition to non-race specificity, may be a component of durable resistance. The response in resistant pi21 plants after pathogen attack is not as fast or as strong as the R gene response. This slower induction of defense may be another type of incompleteness that contributes to the durability of resistance in rice. This report indicated that QTLs conferring incomplete field resistance to rice blast were firstly identified and one of QTLs, pi21, caused by a loss-of-function mutation, has potential for improvement of field resistance to rice blast. The durability of a resistance gene needs to be proved when cultivars carrying that gene alone maintain prolonged resistance under natural field conditions. Monitoring of newly released cultivars provides further evidence to confirm the durability of pi21-mediated resistance in rice. Moreover, pi21 has been used for upland rice breeding in Africa Rice Center and its durability will be monitored in rice cropping areas in Africa.

References