Bovine Isolated Abomasum Specimens - A Useful Tool in Preclinical in vitro Studies
To obtain repeatable and reliable results in preclinical studies based on cow isolated abomasum specimens, it is necessary to confirm the stability of the model in long-term experiments conducted on the day of tissue collection and after cold storage.
The experiments were conducted on tissue samples containing abomasum longitudinal and circular smooth muscles that were collected from routinely slaughtered animals. The dose-response curves for acetylcholine and carbachol were determined. The stability of the response of abomasum strips to acetylcholine was verified during long-term incubation and after 24 and 48 h cold storage. The morphological stability of specimens was analysed microscopically after staining with haematoxylin and eosin.
Acetylcholine and carbachol generated concentration-dependent contraction of longitudinal and circular abomasal preparations. The reactivity to the reference contractile substance, acetylcholine, was stable through 4.5 h of incubation on the day of tissue collection and after cold storage for 24 or 48 h. The morphology of smooth muscles is only slightly affected after long-term incubation and remains unaffected after 24 h cold storage.
The results of the performed study indicate clearly that the model of bovine isolated muscle specimens from abomasum can be a useful tool in preclinical in vitro studies.
Mendel M, Chopecka M, Dziekan N, Sobczak-Filipiak M, Bielecki W, Karlik W