In vivo anticandidal Activities of Sub-fraction S (237-253) From Uvariondendron Calophyllum Ethanolic Leaf Extract
Background and objectives: Mucosal candidiasis can disseminate through the bloodstream and cause disseminated candidiasis. The acute toxicity and the effect of sub-fraction S (237-253) on the vaginal and systemic candidiasis in BALB/C mice model was evaluated.
Methodology: The leaf crude extract of Uvariodendron calophyllum was prepared using Ethanol 95% then partitioned using hexane, methylene chloride and methanol and the methylene chloride fraction was fractionated using column chromatography to obtain sub-fraction S (237-253). Vaginal and systemic candidiasis were induced using Candida albicans strains NR- 29445 and NR-29450 on immunocompromised BALB/C mice model and treated with subfraction S at 100, 200 and 400 mg/kg bw. The number of colony forming units in the vaginal candidiasis on the vaginal smears was measured by culturing the vaginal smears on SDA during ten days of treatment. In the systemic candidiasis, the number of colony forming units in the blood, spleen, kidney, liver and lungs of the different groups of mice was cultured with their histopathological analysis evaluated.
Results: The results show that LD50 was > (2000 mg/kg). The healing effect was observed with the different doses (100 mg/kg, 200 mg/kg and 400 mg/kg) and was dose dependent. Inflammation of the stratified squamous epithelium (increased size) in the treatment with water 2 ml/100 g indicating the level of infection and progression was observed. The treatment with sub-fraction S at 400 mg/kg reduced the size of the stratified squamous epithelium. The healing effect was dose dependent for the different organs.
Conclusion: The sub-fraction S could be potential source of constituents with in-vivo anticandidal properties with little or no side effect.
Zeuko’o Menkem Elisabeth, Toghueo Kouipou Rufin Marie, Siwe Tchokomeni Gael, Ngouana Kammalac Thierry, Fekam Boyom Fabrice