31 / 42
Page 142
conferenceseries
.com
Volume 6
Research & Reviews: Journal of Material Sciences
ISSN: 2321-6212
Advanced Materials 2018
September 04-06, 2018
September 04-06, 2018 | Zürich, Switzerland
21
st
International Conference on
Advanced Materials & Nanotechnology
A new interfacial bio-sensing approach for detecting aberrant protein phosphorylation in cancer
Mostak Ahmed, Laura G Carrascosa, Paul Mainwaring
and
Matt Trau
University of Queensland, Australia
P
rotein phosphorylation is one of the most prominent post-translational mechanisms for protein regulation, which is
frequently impaired in cancer. Through the covalent addition of phosphate groups to certain amino-acids, the interactions
of former residues with nearby amino-acids are drastically altered, resulting in major changes of protein conformation that
impacts its biological function. Herein, we report that these conformational changes can also disturb the protein’s ability
to interact with and adsorb onto bare gold surfaces. Based on the direct interaction of proteins with the gold interface, we
further developed an extremely simple method for aberrant phosphorylation detection that circumvents the current need
for phospho-specific antibodies. The novel interfacial bio-sensing method, which only requires 50 ng of purified protein, was
applied to EGFR phosphorylation analysis in several lung cancer cell lines and enabled monitoring their cell sensitivity to
tyrosine kinase inhibitors (TKI) a drug frequently used in the clinic for lung cancer treatment.
m.ahmed2@uq.edu.auRes. Rev. J Mat. Sci. 2018, Volume 6
DOI: 10.4172/2321-6212-C3-021