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Volume 08
June 28-29, 2019 | Oslo, Norway
Joint Event
Journal of Medical and Health Sciences | ISSN : 2319-
Breast Pathology and Cancer, Gynecology and Obstetrics Pathology, Palliativecare and Gerontology 2019
Breast Pathology & Cancer
Palliativecare & Gerontology
Gynecology and Obstetrics Pathology
Hsa_circ_0000376: A potential novel biomarker for the prediction of
primary ovarian insufficiency
Background:
Primary ovarian insuff iciency (POI) is a reproductive disorder
occurs to women before 40 years old, which affects their fertility and life quality.
However, the mechanisms of POI remain largely unknown, approximately 50%
accordingly. Circular RNAs (circRNAs) have recently been implicated in ovarian
aging, but their potential as biomarkers was never investigated in POI. Herein,
this study aimed to identify the role of circRNAs as a potential biomarker in POI
patients.
Methods:
CircRNA expression screening was performed in plasma RNA from
f ive pairs of POI patients and age- and sex-matched controls using circRNA
microarray. In the validation phase, 18 circRNA candidates were validated by
quantitative reverse transcription polymerase chain reaction (qRT-PCR) in a
cohort of 25 patients diagnosed with POI and 25 age- and sample storage time-
matched controls.
Results:
Of 6644 differentially expressed circRNAs, 12 signif icantly up-
regulated and 23 signif icantly down-regulated circRNAs were identif ied in the
POI patients compared with the control group, which showed as red points in
the volcano plot analysis (p<0.05, fold change>1.5). Based on the evaluation
of initial expression quantity and fold change values, 18 circRNAs candidates
were selected for the validation phase. Hsa_circ_0000376 was significantly
downregulated in POI patients compared with the control group in qRT-PCR (P
<0.05). The area under the ROC curve (AUC) of hsa_circ_0000376 was 0.698
(95% CI 0.499-0.897). The sensitivity and specificity at the cutoff value of 0.685
were 73.3% and 60%, respectively.
Conclusion:
Taken together, our study indicates that hsa_circ_0000376 may
play potential roles in predicting POI.
Biography
Ying Zhao has completed her PhD, is the Professor of Obstetrics and Gynecology in the
Guangzhou University of Chinese Medicine. She is also the Director of the Center for Women’s
Health Research and an expert in using Chinese Medicine as the treatment for Reproductive
Disorders. She has published 49 papers in reputed journals, which focused on her specialties:
Reproductive Endocrinology and Infertility, Gynecology. She has been the Leader in several
research programs, which funded by the National Natural Science Foundation of China.
supersuccessmaria@hotmail.comYihui Feng, RRJMHS 2019, Volume 08
Yihui Feng
Guangzhou University of Chinese Medicine,
China