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Research & Reviews: Journal of Material Sciences | ISSN: 2321-6212 | Volulme 6
November 07-08, 2018 | Atlanta, USA
Materials Science and Engineering
15
th
International Conference and Exhibition on
Applied Crystallography
3
rd
International Conference on
&
Serial crystallography of a G-protein coupled receptor using polychromatic synchrotron radiation
source
Ming Yue Lee
Arizona State University, USA
S
ince the first successful serial crystallography (SX) experiment at synchrotron radiation sources, the popularity of this
approach has continued to grow, showing that 3
rd
generation synchrotrons can be viable alternatives to scarce X-ray free
electron laser sources. Synchrotron radiation flux may be increased about 100 times by a moderate increase in bandwidth
(“pink beam” conditions) at some cost in data analysis complexity. Here, we report the first high-viscosity injector-based pink
beam SX experiments. The structures of A
2A
adenosine receptor (A
2A
AR) and proteinase K (PK) were determined to 4.2Å and
1.8Å resolution using 24 and 4 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using
existing Laue approaches, while weaker A
2A
AR required an alternative data processing strategy. This demonstration of the
feasibility presents new opportunities for the time-resolved experiments with micro-crystals to study structural changes in
real-time at pink beam synchrotron beamlines worldwide.
Biography
Ming Yue Lee is an expert in macromolecular crystallography with focus on technology development and implementation in novel membrane protein crystallization
and diffraction methods. He has made contributions in the field of GPCR structural biology in the forms of active participation and validation of serial femto-second
crystallography using XFEL sources, as well as being involved in validation of delivery mechanisms for various cutting-edge diffraction experiments both at
XFEL and synchrotron radiation sources. He is actively leading and driving the effort to develop and implement technology that can enhance and optimize serial
crystallography at polychromatic synchrotron radiation sources. His current focus is building up a system approach to study membrane protein structure-function
relationships between different components of the cellular membrane environment with an emphasis on spatial and temporal resolution of proteomic interactions.
minglee@asu.eduMing Yue Lee, Res. Rev. J Mat. Sci. 2018, Volume 6
DOI: 10.4172/2321-6212-C8-035