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Research & Reviews: Journal of Material Sciences | ISSN: 2321-6212 | Volulme 6
November 07-08, 2018 | Atlanta, USA
Materials Science and Engineering
15th International Conference and Exhibition on
Applied Crystallography
3rd International Conference on
&
Transient expression of monoclonal antibody and bispecific fragments and fragment/antigen
complexes for pharmaceutical discovery research
Ramesh Iyer
AbbVie Inc., USA
Fragments, such as F(ab) and F(ab’)2 and fragment complexes are widely required for various research and development
activities in biopharmaceutical discovery. We describe the production of fragments (F(ab) and F(ab’)2) from a variety
of parent molecules (monoclonal antibody and DVD-Ig) and frameworks (mIgG1, mIgG2a, huIgG1, huIgG4) by transient
expression inHEK293 cells.Themethod eliminates the need touse enzyme digestionof have alsodeveloped
a route for the production of fragment/antigen complexes by direct co-expression in HEK293 for crystallization purpose
that allows for higher throughput screening of F(ab) and/or antigen constructs. The resulting products were characterized
by biophysical techniques and crystallization experiments demonstrated that the fragments and fragment/antigen complexes
produced diffraction quality crystals suitable for X-ray crystallographic analysis.
Biography
Ramesh Iyer has completed his PhD from the University of Kentucky (Lexington, KY) and Postdoctoral studies from the University of Georgia (Athens, GA). He
is a Sr Scientist in the Global Biologics group at AbbVie Inc. His group supports the research and development of biologics for various therapeutic areas within
AbbVie Discovery.
Ramesh Iyer, Res. Rev. J Mat. Sci. 2018, Volume 6
DOI: 10.4172/2321-6212-C8-035