ISSN: 1204-5357

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

RESPONSE OF LEAVES, STEMS AND ROOTS OF WITHANIA SOMNIFERA TO COPPER STRESS

Visit for more related articles at Journal of Internet Banking and Commerce

Abstract

The effects of Cu+2 stress (0, 10, 20, 50, 100 and 200 mM as CuSO4.5H2O) on growth and biochemical parameters of Withania somnifera L.Dunal, grown in a field pot experiment were studied. Increasing Cu+2 concentrations led to decreased stem length, root elongation and leaf area. Copper (Cu+2 ) stress decreased chlorophyll and carotenoids content in leaves and stems as compared to controls; this was more pronounced in leaves than in stems. Lipid peroxidation, in terms of malondialdehyde (MDA) content increased. Lipid peroxidation increased significantly with elevation in Cu+2 concentrations and was highest in roots followed by leaves and stems at 200 mM. Leakage of electrolytes measured in terms of membrane stability index (MSI), which showed a negative correlation with increasing Cu+2 levels. The MSI was lowest in 200 mM Cu+2 treated plants. The phenol content of leaves, stems and roots peaked at 50 mM Cu+2 treatment and thereafter declined. These results indicate that Cu+ 2 stress negatively impacted nearly all the parameters assayed; particularly in the roots. It is being reported for the first time that stress resistance in W.somnifera as observed by increase in total phenol content at low Cu+2 levels possibly to combat increased ROS generation.