|Pranay Abhang1,3, Girish Pathade2,3
|Related article at Pubmed, Scholar Google|
Visit for more related articles at International Journal of Innovative Research in Science, Engineering and Technology
The purpose of this study was to screen scytonemin, a UV screening pigment, in isolated Nostocales, Scytonema sp., Plectonema sp., Spirulina sp., and Lyngby sp. Scytonemin was isolated by silica gel column chromatography and its effects were tested in vitro for growth profile and melanogenesis on B16F10 mouse melanoma cell line. In order to study effect of these scytonemin on growth profile- cell viability count, MTT and DCPIP cytotoxicity assay, Comet genotoxicity assay and microscopic observations were considered. To study tyrosinase enzyme activity- diphenolase assay and native SDS PAGE methods were used. Scytonemin isolated from Scytonema sp. and Lyngby sp. shows decrease in melanogenesis, which inhibit tyrosinase activity, having 30.25 μM, 100.25 μM IC 50 values respectively. While that of scytonemin isolated from Plectonema sp. and Spirulina sp. was not showing effect on melanogenesis as tyrosinase activity not altered, having 45.5 μM, 60.5 μM IC 50 values respectively
|Cytotoxicity, Genotoxicity, Melanogenesis, Scytonemin, Tyrosinase.|
|For continued existence, solar radiation plays important role in evolution and development of life. Solar radiation that reaches to the EarthÃ¢ÂÂs surface, the ultraviolet (UV) radiation is of particular importance for the human skin. Skin pigmentation i.e. production of melanin by the process known as melanogenesis is highly depend on solar UV radiations (Del Marmol et al., 1993, Jimbow etb al., 2000). At molecular level tyrosinase enzyme regulates process of melanogenesis in response to UV rays, in which it act as key enzyme and regulate conversion of tyrosine to DOPA and DOPA to DOPAquinone. (Hearing V. J. et al., 1980, Hearing and Tsukamoto, 1991, A. slominski et al., 2004).|
|The UVÃ¢ÂÂregion of the electromagnetic spectrum is divided into three different regions: UVC radiation (200Ã¢ÂÂ290 nm), UVB radiation (290Ã¢ÂÂ320 nm) and UVA radiation (320Ã¢ÂÂ400 nm). Of these wavelengths all UVC radiation as well as large quantities of the UVB radiation gets absorbed by ozone layer. Therefore, the UV radiation that reaches the EarthÃ¢ÂÂs surface contains about 5% of UVB and 95% of UVA radiation. (Mang R. et al., 2006)|
|Increased penetration of UV-B (not of UVA) on the surface of the earth is due to the decreasing ozone concentration in the stratosphere (Kerr et al., 1993 and S. Madronich et al., 1998). Thus UV-B, which is deemed deleterious, can penetrate biologically significant depths into the water column (Kuwahara et al., 2000), thereby affecting aquatic ecosystems. The detrimental effects of UV-B on primary producers are varied, mediated primarily by damaging molecular targets such as nucleic acids, proteins and pigments, and indirectly, by producing reactive oxygen species. These can lead to the inhibition of cell division and growth, affecting various physiological and biochemical processes, such as motility, orientation in motile organisms and photosynthesis, which can consequently alter community structure and function. UV-A can have a net damaging influence on photosynthesis (Cullen et al., 1992).|
|Although UV rays are harmful, some organisms attempt to cope with UV radiation. Sinha et al. (1998) identified the following four adaptation strategies through organisms tolerate or adapt to UV environment -|
|I. DNA repair mechanisms|
|II. Production of enzyme systems and induced formation of quenching agents|
|III. Behavioral modification to avoid exposure to UV|
|IV. Production of UV-absorbing substances|
|In terrestrial environments, where higher plants are the foremost primary producers, several studies have shown that harmful UV radiation in higher plants is absorbed by epidermally located phenylpropanoids, mainly flavonoid derivatives (Kootstra, 1994). In aquatic environments, where microalgae found abundantly, the presence of UVabsorbing compounds like sporopollenin, scytonemin, and mycosporine-like amino acids (MAAs) have been established.|
|Scytonemin [(3E,3'E) -3,3'-bis(4-hydroxybenzylidene) - [1,1'- bi(cyclopenta [b] indole)] -2,2' (3H,3'H) –dione] is a UVÃ¢ÂÂscreening pigment that is commonly produced in populations of sheathed cyanobacteria that live in different habitats and geographic locations, where solar radiations are very intense (Garciapichel et al., 1991) The yellow-green pigment scytonemin was first reported by Nageli as early as 1849 and the chemical structure was provided by Proteau et al., 1993. It is a lipid soluble alkaloid that is synthesized in response to UVA radiation and accumulates within the extracellular sheaths of cyanobacteria. The organisms are thereby protected from cell damage by this natural UVÃ¢ÂÂfilter that absorbs the harmful solar radiation. (Fleming and Castenholz, 2007 and Stevenson et al., 2002) Scytonemin absorbs mostly in the UVA (325Ã¢ÂÂ425 nm, λmax = 370 nm) and UVC region (λmax = 250 nm), but it also absorbs substantially in the UVB region (280Ã¢ÂÂ320 nm). The maximum absorption wavelength of scytonemin is 370 nm in vivo. However, it shifts towards a longer wavelength of 384 nm in a solvent after isolation. It is reported that the molar extinction coefficient of scytonemin is large (250 l/g/cm) at wavelength 384 nm (Vincent et al., 1993), it is calculated to be 136,000 l/mol/cm based on a molecular weight of 544 Da. Therefore, scytonemin is an efficient photo-protective compound due to its large extinction coefficient (Bultel-Poncef et al., 2004). It is a dimer composed of indolic and phenolic subunits having a molecular mass of 544 g/mol. The linkage between two subunits in scytonemin is an olefinic carbon atom that is unique among natural products. Hence, scytonemin possess a new ring system in nature for which Proteau et al. (1993) have proposed the trivial name „the scytoneman skeletonÃ¢ÂÂ. Scytonemin exists in oxidized (green) and reduced (red) form. In an oxidized state, the two chromophores are connected by a single bond. Therefore, they can freely rotate and prevent steric repulsion. This steric repulsion between two bulky chromophores makes a dihedral angle of about 90 degrees, so electronic interaction between them becomes very weak. On the other hand, in a reduced state, the indole ring and the benzene ring, which have 10-π and 6-π electron aromaticity, respectively, are alternately connected by double bonds. Therefore, π-conjugation is expanded by increase of planarity. Due to this structural and electrical change, the color of the compound changes from brown (oxidized state) to red (reduced state) (Saman M. et al., 2014).|
|During this study naturally occurring UV screening compound is isolated from Nostocales and tested in vitro for melanogenesis using mouse melanoma cells.|
|1. Isolation of cyanobacteria|
|A. Collection and Enrichment of samples|
|Water and Soil samples were collected from Old swimming pool in Savitribai Phule Pune University, in autoclaved glass bottle. Algal blooms or mats were collected by using mesh net (pore size 25-30 μm). pH and salinity of samples were recorded using pH strips and salinometer respectively. Samples were centrifuged and pellet was suspended in BG 11 media with 1 mg/L Germanium Dioxide and 0.05 mg/ml Cycloheximide. Samples were kept for enrichment by providing 12 hours light and dark conditions at 28oC for 10-15 days. Bacterial and fungal contamination was reduced by adding 0.05 mg/L streptomycin, 10 U/ml penicillin-G, 0.05 mg/L amphotericin B in the media. (Algal culture techniques, by Robert Andersen)|
|B. Preparation of Axenic culture|
|Unialgal cultures were obtained by using serial dilution method for enriched samples. Enriched samples were diluted as 10-2 - 10-10 in BG 11 media and spread on BG 11 agar plates (1.5% agar) and incubated for 10-15 days by providing 24 hours light condition at 28oC. Green colored colonies were streak on fresh BG 11 agar plates (1.5% agar with 5 μg/L streptomycin, 10 U/ml penicillin-G, 5 μg/L amphotericin B in BG 11 media). Plates were incubated for 15-20 days by providing 24 hours light condition at 28oC. Mixed algal culture was separated under stereo microscope. (Algal culture techniques, by Robert Andersen)|
|C. Identification of cyanobacteria|
|Isolated cultures were identified by colony characteristics, presence of pigments (and), cellulose and pectin, microscopic observations as per given in “The freshwater algal flora of the British Isles: an identification guide to freshwater and terrestrial algae.” edited by D. M. John et al. (2002) and “Cyanophyta” by T. V. Desikachary. 80% acetone extract of sample was used to detect presence of β-carotene (450 nm) and chlorophyll a (430 nm and 662 nm) while that of phosphate buffer extract of sample was used to detect presence of phycocyanobillin (620 nm 652 nm or 562 nm). Presence of cellulose and pectin in sample was detected by Anthrone method (E. P. Samsel and J. C. Aldrich, 1957) and Carbazole test (Mc Comb et al., 1957 and S. Nurdjanah et al., 2013) respectively.|
|2. Screening of scytonemin from cyanobacterial isolates|
|Presence of scytonemin was detected by following techniques (Proteau et al., 1993).|
|A. TLC for scytonemin|
|5 ml acetone was added in 10 mg of cyanobacterial sample and kept in dark at 4oC for about 10-12 hours. 0.1 μl of sample was spotted on TLC plates (Sigma-Aldrich, silica coated on aluminum support with pore size of 60 Ao and thickness of 200μm) and run by using chloroform: methanol (9: 1) as mobile phase. Yellowish brown colored spot was detected and Rf value was calculated. (Rf value for scytonemin is around 0.8)|
|B. Spectrophotometric analysis|
|5 ml acetone was added in 10 mg of cyanobacterial sample and kept in dark at 4oC for about 10-12 hours. Sample was centrifuged at 5000 rpm for 10 min at 4 oC and supernatant was scanned from 350 nm to 450 nm on spectrophotometer (Shimadzu UV 1601 UV-Visible). Peak at 384 nm (or 380nm -390 nm) shows presence of scytonemin. (Shailendra et al. 2010). Scytonemin containing cyanobacteria were used for further experimentations.|
|3. Isolation of Scytonemin|
|Biomass was oven dried at 60 0C. Dry weight was recorded and scytonemin was isolated by column chromatography. (Naoki W., 2013). About 2.5 gram of dried biomass were suspended in 50 ml of acetonitrile and kept at 4 0C for 1 hr. biomass was separated by centrifugation and supernatant containing pigments was collected. This step was repeated twice. Acetonitrile was removed by using Rota-vapor (Buchi R-210). Dried pigments were suspended in 50 ml of ethyl ether to remove chlorophyll and other pigments. Un-dissolved pigments were collected and dissolved in acetone. Silica gel (Hi-media) column was prepared in acetone and samples were resolved on 50 cm silica column using acetone as mobile phase. Procedure was repeated twice for further purification. Collected samples were screened on spectrophotometer (Shimadzu UV 1601) for scytonemin.|
|4. Study effect of scytonemin on growth profile and melanogenesis|
|A. Cell line maintenance -|
|B16F10 mouse melanoma cell line was purchased from NCCS, Pune. Cell line was maintained in DMEM with 10% serum and 50 μg/ml streptomycin by providing 5% of CO2 atmosphere. Sub-culturing was done after every 5 days (Culture of Animal cells by Ion Freshney).|
|B. Addition of scytonemin-|
|Purified scytonemin stock 10 mM (5.5 mg/ml) was prepared in 100 % acetone. For testing, 1μm, 10 μm, 100 μm, 1000 μm and 10000 μm concentration was added in cell culture (initial cells concentration was 3 × 105 cells/well).|
|C. Cell viability count-|
|Cell viability was counted with the help of 0.4% Trypan blue vital stain using haemocytometer. Stained and nonstained cells counted under microscope and recorded as cells/ml. (Culture of Animal cells by Ion Freshney)|
|D. Protein estimation|
|Protein estimation was done by modified BradfordÃ¢ÂÂs assay (1976). Unknown proteins were estimated by dissolving cell pellets in protein extraction buffer containing 50 mM Tris-HCl, 5 mM EDTA, 0.1% Triton X-100 and 1 mM PMSF. 0.2 ml of 5x BradfordÃ¢ÂÂs reagent (500 μg/ml CBB G-250, 25% of ethanol and 50% of ortho-phosphoric acid) were added in 0.8 ml diluted sample by vortexing and OD was taken at 595 nm. OD was compared with BSA standard graph and unknown concentrations of proteins were estimated.|
|E. Tyrosinase enzyme activity staining in situ on SDS polyacrylamide gel –|
|Assay was done as described by Jimenez-Cervantes et al. (1993). Cell pellets were lysed in lysis buffer containing 50 mM TrisHcl, 5 mM EDTA, 0.1% Triton X-100 and 1 mM PMSF. The melanoma protein samples were mixed with sample buffer without β-mercaptoethanol (0.18 M Tris- HCl pH 6.8, 15% glycerol, 0.075% bromophenol blue, 9% SDS) in a 2:1 ratio without giving heat treatment. The samples were then resolved by 12% SDS-PAGE at 30mA. After electrophoresis gel were equilibrated in 50 mM sodium phosphate buffer (pH 6) for 15 min at 37oC. Tyrosinase activity was detected by immersing the equilibrated gel in 50 mM sodium phosphate buffer (pH 6.8) containing 1.5 mM LDOPA and 4 mM MBTH at 370C with gentle shaking until dark pink-brown coloured bands were developed.|
|F. Tyrosinase enzyme assay-|
|The tyrosinase activity was determined by a spectrophotometric method, as described by (Celia et al. 2001). The 10 μl enzyme extract was added to the substrate containing 1.5 mM L-DOPA, 1 mM DMF and 4 mM MBTH in phosphate buffer (pH 6.8). L-DOPA is the main substrate that converts to dopaquinone by enzyme activity. MBTH is a strong nuocleophile that is attached to produce a pink complex. DMF is added to the reaction mixture in order to keep the resulting complex in solution state during the course of investigations. The progress of the reaction was followed by measuring the intensity of the resulting pink color at 505 nm. A typical reaction mixture with a total volume of 1 ml contained 100 μl enzyme solution, 850 μl substrate solution and 50 μl phosphate buffer (pH 6.8). To estimate tyrosinase activities in, the molar extinction coefficient of the product was 3700 per mole per centimetre.|
|G. Superoxide dismutase (SOD) assay|
|SOD assay was done as given by Adriana M C et al. (2004). Enzyme extract was prepared by dissolving cell pellet (containing 103 cells/ml) in lysis buffer and supernatant were used after centrifugation at 12000 rpm. Following components were added in two sets of tubes. 1.5 ml of 0.1 M potassium phosphate buffer (pH 7.8), 0.6 ml of 65 mM L- methionine, 0.3 ml of 750 μM NBT, 0.3 ml of 2 mM Riboflavin, 1μl of 10 mM EDTA, 0.1 ml of Enzyme extract (for control 0.1 ml water was added) and volume was adjusted to 3.5 ml with Distilled water. One set of tube was illuminated under the light source for 30 min at a distance of 30 cm and another set of tube was kept in dark for 30 min. Absorbance was read at 560 nm after 30 min of incubation at room temperature. Amount of SOD is directly proportional to the amount of reactive oxygen species generated during reaction which are measured at 560 nm and calculated by formula.|
|H. MTT and DCPIP assay for cell cytotoxicity|
|Cell cytotoxicity was tested as given by Karl B. et al. (2012). On 1st day, 1 x 105 cells/well allow to attach in 96 well plate with 200 μl of complete medium in CO2 incubator.|
|On 2nd day, compounds to be tested was added in respective wells with fresh medium and incubated for 24 hr.|
|On 3rd day, medium were removed and monolayer were washed with PBSA 3-4 times.|
|For MTT assay - Fresh medium containing 5 μg/ml MTT were added in each well and incubated for 4 hr in CO2 incubator.|
|After 4 hr. medium was removed and crystals were dissolved in 200 μl of DMSO. To this 50 μl of glycine buffer (0.1 M glycine, 0.1 M NaCl, pH 10.5) was added.|
|Immediately absorbance at 570 nm was recorded using ELISA plate reader. And IC 50 value was calculated.|
|For DCPIP assay - 200 μl fresh medium were added in each well, to this 5 μl of 2 mM DCPIP reagent is added in each well and incubated for 4 hour in CO2 incubator.|
|After 4 hr. absorbance at 660 nm was recorded using ELISA plate reader. And IC 50 value was calculated.|
|I. Comet assay for genotoxicity|
|Assay was performed as given by Stevenson C. S. et al. (2002). Glass slide is covered with thin layer of 1.5% melted agarose. To the 200 μl of 0.6% melted agarose total of 20μl of cell suspension (1x106/ml) was added. This was spread on coated slide using coverslip. Slide was kept at 0 0C for 2-3 min. and slide was submerged in lysis buffer (0.25 M NaCl, 0.6 % NaOH, 100 mM EDTA, 2% DMSO, 0.2% Triton X 100, pH 10) for 2 hours in dark. Ice cold running buffer (300 mM NaOH, 0.5 M EDTA, pH 13) was added in electrophoresis tank and sample was run at 300 mA for 30 min in dark conditions.|
|After electrophoresis, slide was kept in neutralization buffer (0.4 M tris HCl pH 7.5) and then washed with distilled water. EtBr stain was sprinkled on slide and slides were observed under fluorescence microscopy for comets.|
RESULTS AND DISCUSSION
|1. Isolation of Nostocales and scytonemin|
|11 algae were isolated from collected samples, among those only 6 were cyanobacteria. All 6 cyanobacteria were characterized and screen for scytonemin, only four of them shows presence of scytonemin namely scytonema sp., Plectonema sp., Spirulina sp. and Lyngbya sp. (Fig. 1) Separation of scytonemin on TLC shows Rf values as 0.82 (green) for Scytonema sp., 0.80 (brownish purple) for Plectonema sp., 0.82 (brown) for Lyngbya sp., 0.84 (yellowish brown) for Spirulina sp. and peak at 384 nm. Due to presence of variable side chains and electric charge on Scytoneman skeleton, scytonemin can exist in different colors in nature. As Scytonemin pigment is highly hydrophobic and exist in different color, it can be used in textile industries for coloration.|
|Major role of scytonemin in cyanobacteria is to protect cells from UV rays. As it is a natural UV screening compound having radical scavenging activity, it can be used in cosmetic industries. As per our results scytonemin isolated from Scytonema sp. and Lyngbya sp. inhibit tyrosinase activity, hence it can be used to treat disorders related to melanogenesis such as – Melisma, over pigmentation and deposition of melanin in patches. As tyrosinase activity inhibited by scytonemin it may regulate melanin biosynthesis and hence melanin production can be regulated.|
|Authors are thankful to Dr. R. G. Pardeshi, Principal Fergusson College, Pune for provision of laboratory and chemicals.|
| A. Slominski, Desmond J. Tobin, S. Shibahara, and J. Wortsman. Melanin Pigmentation in Mammalian Skin and Its Hormonal Regulation. Physiol Rev. 2004; 84:1155-228.
 Adriana M. Carvalho, Ana M. P. Neto, Angela P. Tonon, Ernani Pinto, Karina H. M. Cardozo, Maisa RPL Brigagao, Marcelo P. Barros, Moacir Aluisio Torres, Paula Magalhães, Sara C. G. Campos, Thais Guaratini, Teresa C. S. Sigaud-Kutner, Vanessa R. Falcao and Pio Colepicolo. Circadian protection against oxidative stress in marine algae, Hypnos. 2004; 1 (Suppl 1): 142-57.
 Algal Culturing Techniques, Edited by Robert A. Andersen, Elsevier Academic Press 2005 - ISBN: 0-12 088426-7.
 Bradford M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal Biochem. 1976; 72, 248–54.
 Bultel-Ponce V, Felix-Theodose F, Sarthou C, Ponge J F, Bodo B., New pigments from the terrestrial cyanobacterium Scytonema sp. collected on the Mitaraka inselberg, French Guyana. J Nat Prod. 2004; 67(4):678-81.
 Celia Jimenez-Cervantes, MariaMartinez-Esparza, Cristina Perez, Nicole Daum, Francisco Solano and Jose Carlos Garcia-Borron. Inhibition of melanogenesis in response to oxidative stress: transient downregulation of melanocyte differentiation markers and possible involvement of microphthalmia transcription factor. Journal of Cell Science. 2001; 114, 2335-44.
 Cullen, J. J., Neale, P. J., Lesser, M. P. Biological weighting function for the inhibition of phytoplankton photosynthesis by ultraviolet radiation. Science. 1992; 258, 646-50.
 Culture of animal cell, a manual of basic techniques. By Ion Freshney.
 Cyanophyta by T. V. Desikachary. Published by Indian Council of Agricultural Research, New Delhi (1959).
 Del Marmol V, Beermann F. Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett. 1996; 381:165-8.
 E. P. Samsel, J. C. Aldrich. Application of Anthrone Test to Determination of Cellulose Derivatives in Nonaqueous Media. Anal. Chem., 1957; 29 (4), 574-6.
 Fleming, E. D., and Castenholz, R. W. Effects of periodic desiccation on the synthesis of the UV-screening compound, scytonemin, in cyanobacteria. Environmental Microbiology. 2007; 9, 1448-55.
 Garciapichel, F., and Castenholz, R. W. Characterization and biological implications of scytonemin, a cyanobacterial sheath pigment. Journal of Phycology. 1991; 27, 395-409
 Hearing V j, Tsukamoto K. Enzymatic control of pigmentation in mammals. FASEB J. 1991; 5:2902-9.
 Hearing V. J., Ekel T. M., Montague P. M., Nicholson J. M. Mammalin tyrosinase. Stoichiometry and measurement of reaction products. Biochim. Biophys. Acta. 1980; 611 (2): 251–68.
 J. B. Kerr, C. T. McElroy, Evidence for large upward trends of ultraviolet-B radiation linked to ozone depletion, Science. 1993; 262 (5136), 1032-34.
 Jimbow K, Hau C, Gomez PF, Hirosaki K, Shinoda K, Salopek T G, et al. Intracellular vesicular trafficking of tyrosinase grne family protein in eu and pheomelanosome biogenesis. Pigment Cell Res 2000; 13(Suppl.8), 110-7.
 Jimenez-Cervantes C, Valverde P, Garcia-Borron J C, Solano F, Lozano JA. Improved tyrosinase activity stains in polyacrylamide electrophoresis gels. Pigment cell Res 1993; 6, 394-9.
 John D. M., B. A. Whitton and A. J. Brook. (2002). The freshwater algal flora of the British Isle: an identification guide to freshwater and terrestrial algae. Cambridge University Press. New York.
 Karl Buch, Tanja Peters, Thomas Nawroth, Markus Sänger, Heinz Schmidberger, and Peter Langguth. Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay – A comparative study. Radiat Oncol. 2012; 7: 1.
 Kuwahara, V. S., Toda, T., Hamasaki, K., Kikuchi, T. and Taguchi, S. Variability in the relative penetration of ultraviolet radiation to photosynthetically available radiation in temperate coastal waters, Japan. J. Oceanog., 2000; 56, 399-408.
 Mang, R., Stege, H., and Krutmann, J. (2006) Mechanisms of phototoxic and photoallergic reactions, In Contact Dermatitis (Frosch, P. J., Menné, T., and Lepoittevin, J.-P., Eds.) pp 97-104, ISBN: 978-3-662-10304-3 Springer-Verlag, Berlin Heidelberg.
 Naoki Wada, Toshio Sakamoto and Seiichi Matsugo. Multiple Roles of Photosynthetic and Sunscreen Pigments in Cyanobacteria Focusing on the Oxidative Stress. Metabolites. 2013; 3, 463-83.
 Proteau, P. J., Gerwick, W. H., Garciapichel, F., and Castenholz, R. The structure of scytonemin, an ultraviolet sunscreen pigment from the sheaths of cyanobacteria. Experientia. 1993; 49, 825-9.
 S. Madronich. Implications of recent total atmospheric ozone measurements for biologically active ultraviolet radiation reaching the Earth’s surface, Geophys. Res. Lett. 1992; 19, 37–40.
 S. Nurdjanah, James Hook, J. Paton and J. Paterson. Galacturonic Acid Content and Degree of Esterification of Pectin from Sweet Potato Starch Residue Detected using 13C CP/MAS Solid State NMR. European Journal of Food Research & Review, 2013; 3(1), 16-37.
 Saman Mushir, Satyanarayan Deep, Tasneem Fatma Screening of cyanobacterial strains for UV screening compound Scytonemin – Environmental Perspectives International Journal of Innovative Research in Science, Engineering and Technology. 2014; Vol. 3, Issue 2, ISSN: 2319-8753.
 Shailendra P. Singh, Sunita Kumari, Rajesh P. Rastogi, Kanchan L. Singh, Richa and Rajeshwar P. Sinha, Photoprotective and biotechnological potentials of cyanobacterial sheath pigment, scytonemin. African Journal of Biotechnology. 2010; Vol. 9(5), pp. 580-8.
 Shazia Suhail, Deboshree Biswas, Alvina farooqui, J. M. Arif and Mohd. Zeeshan, Antibacterial and free radical scavenging potential of some cyanobacterial strains and their growth characteristics. J. Chem. Pharm. Res. 2011; 3(2):472-478.
 Sinha R. P., Klisch M., Gröniger A., Häder D. P. Responses of aquatic algae and cyanobacteria to solar UV-B. Plant. Ecol. 2001; 154, 219–36.
 Sinha R. P., Klisch M., Gröniger A., Häder D. P. Ultraviolet absorbing screening substances in cyanobacteria, phytoplankton and microalgae. J. Photochem. Photobiol. B: Biol. 1998; 47: 83-94.
 Stevenson, C. S., Capper, E. A., Roshak, A. K., Marquez, B., Eichman, C., Jackson, J. R., Mattern, M., Gerwick, W. H., Jacobs, R. S., and Marshall, L. A. Scytonemin – a marine natural product inhibitor of kinases key in hyperproliferative inflammatory diseases. Inflammation Research, 51, 112-114. The identification and characterization of the marine natural product scytonemin as a novel antiproliferative pharmacophore. Journal of Pharmacology and Experimental Therapeutics. 2002; 303, 858-866.
 Takamatsu, S., Hodges, T. W., Rajbhandari, I., Gerwick, W. H., Hamann, M. T., and Nagle, D. G. Marine natural products as novel antioxidant prototypes. Journal of Natural Products. 2003; 66, 605-8.
 Vincent, W.F., Downes, M.T., Castenholz, R.W., Howard-Williams C. Community structure and pigment organization of cyanobacteria-dominated microbial mats in Antarctica. Eur. J. Phycol. 1993; 28, 213-21.