ISSN ONLINE(2319-8753)PRINT(2347-6710)

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Dynamic Assessment fo Biocidal Properties of Silicon-Chitosan -Containing Hydrogels

Alzubaidi Adawia Fadhel Abbaas1,2
  1. PhD Student, Department of Microbiology and Plant Physiology , Fluctuate Biology, Saratov State University, Russia
  2. Senior lecturer, Department of Microbiology, Medical College , Diyala university, Iraq
Related article at Pubmed, Scholar Google

Visit for more related articles at International Journal of Innovative Research in Science, Engineering and Technology

Abstract

Our researches were focused on the study of the possibility to use the Silicon-chitosancontaining hydrogels as the more active antimicrobial substances needed for the manufacture of drugs for the treatment of many infectious diseases. Up until now, its antimicrobial activity is not well researched and its validity remains only vaguely defined.

Keywords

Silicon-Chitosan , Chitosan , Antimicrobial.

INTRODUCTION

Chitin is a polysaccharide of animal origin found abundantly in nature and characterized by a fibrous structure. It forms the basis of the main constituent of the outer skeleton of insects and crustaceans like shrimp, crabs and lobster[1]. The chemical structure of chitin is similar to cellulose, having one hydroxyl group on each monomer substituted with an acetylamine group (Figure 1). The extraction of chitin involves an acid removal of calcium carbonate (demineralization), generally by hot reaction with HCl, HNO3 or HCl, etc., followed by a deproteinization (removal of proteins). This step usually performed by alkaline treatments (e.g. with NaOH)[ 2]. In its extracted crude form, chitin has a highly ordered crystalline structure, is translucent, resilient and quite tough. It has, however, poor solubility and low reactivity.
chitosan depends on its biological origin, molecular weight and degree of acetylation Since chitosan is soluble in diluted acid solutions, films can be readily prepared by casting or dipping, resulting in dense and porous structure[3]. Chitosan film is regarded as biofunctional material, well tolerated by living tissues, particularly applicable as edible coatings to prolong shelf-life and preserve quality of fresh foods In medical field, chitosan films have been tested as curative wound dressing and as scaffolds for tissue and bone engineeringAdditionally the reactive functional groups present in chitosan (amino group at the C2 position of each deacetylated unit and hydroxyl groups at the C6 and C3 positions) can be readily subjected to chemical derivatization allowing the manipulation of mechanical and solubility properties enlarging its biocompatibility [4]. (Fig. 1).
image
Now our researches are focused on the study of the possibility to use the silicon-chitosan-containing gels (Si-Chit- Gels) as the more active antimicrobial substances needed for the manufacture of drugs for the treatment of many infectious diseases and in wound therapy. Up until now, its antimicrobial activity is not well studded and its validity remains only vaguely defined.

MATERIALS AND METHODS

Bacterial strains:
Three well-characterized standard laboratory strains were used in this study: gram-negative Escherichia coli and gram-positive Staphylococcus aureus and Bacillus cereus.
Bacterial killing assay:
Cultures of the indicator strains were incubated separately in the absence (control) and presence of Si-Chit-Gel for a period of 5 h at 37°C.
The delayed growth of the cell suspensions in broth with/without Si-Chit-Gel was monitored as a decrease in optical
image
image

SAFETY

The low toxicity profile of chitosan compared with other natural polysaccharides is another of its many attractive features. It has been reported that the purity of chitosan influences its toxicological profile, yet its safety in terms of inertness and low or no toxicity has been demonstrated by in vivo toxicity studies. Its oral LD50 (median lethal dose) in mice was found to be in excess of 16 g day-1 kg-1 body weight, which is higher than that of sucrose. [10]. Ylitalo and colleagues (2002) reported the absence of significant side-effects following chitosan ingestion in human studies (for up to 12 weeks). However. We were unable to identify any epidemiological studies or case reports investigating the association of chitosan exposure and cancer risk in humans, any carcinogenicity studies on chitosan in animals and any in vitro or in vivo studies evaluating chitosan for mutagenic effects in the available literature. [11].

SENSITIVITY OF MICROBIOLOGY STRAINS TO CHITSAN

Chitosan has several advantages over regular type of disinfectants owing to its broad spectrum of activity. Chitosan has been observed to act more quickly on fungi than on bacteria and activity against typhoid organisms are comparable to the standard antibiotics used in clinical practiceAs discussed this antimicrobial activity has a strong dependence on MW and DA characteristics and also varied according microorganism strains[12]. There are many studies about the minimum inhibitory concentration (MIC) for chitin, chitosan, their derivatives or combination, with different results for different microorganism. MIC is defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight incubation. It is dependent of many factors and the non-standardized procedures make difficult to compare MIC results from author to author. MIC however is useful as a practical indicator of a primary activity against a selected pathogenic microorganism[13] .

INTERACTION OF CHITOSAN WITH THE BACTERIAL CELL SURFACE

The mode of action of cationic antibacterial agents is widely believed to be the interaction with and disruption of the cell envelope. Electron microscopical examinations of various chitosan-treated microorganisms suggest that its site of action is indeed at the microbial cell surface [14].. nature of chitosan, conveyed by the positively It is generally assumed that the polycationic charged —NH3+ groups of glucosamine, might be a fundamental factor contributing to its interaction with negatively charged surface components of many fungi and bacteria, causing extensive cell surface alterations, leakage of intracellular substances, and ultimately resulting in impairment of vital bacterial activities[15].

CLINICAL STUDY RESULTS

Studies have shown that the gel can significantly increase the effectiveness of basic treatment for gingivitis and chronic periodontitis .
The use of the gel was recommended for the treatment of periodontitis in the pre- and postoperative periods and also during maintenance therapy for the early elimination of inflammatory processes in periodontal tissue.
image

CONCLUSIONS

The gram-negative culture of E.coli exhibited delayed growth in broth containing Si-Chit-Gel compared to growth of the microorganism in the absence of Si-Chit
Similar effects of Si-Chit-Gel on bacterium growth were seen with the use of two additional gram-positive strains S.aureus and B.cereus.
Si-Chit-Gel does not lose antimicrobial activity against gram-negative and gram-positive bacteria within two months of storage at 4oC .
Si-Chit-Gel can be recommended for outdoor use in the treatment of the infectious diseases.

ACKNOWLEDGEMENT

The authors would like to thank the Iraqi Ministry of Higher Education and the University of Diyala / College of Medicine to provide an opportunity to study in Russia , and all the members of the Microbiology Unit (Saratov State University ) for many stimulating discussions and for their valuable help and cooperation Especially Supervisor Ksenofontova О.Yu.

References

  1. Alewo O.A., David A., Muhammed T. I.,and Umar R. "Kinetics of Demineralization of Shrimp Shell Using Lactic Acid" Biomacromolecules, 4, p.12-18 (2003).

  2. Assis, O. B. G. & Pessoa, J. D. C. "Preparation of Thin Films of Chitosan for use as Edible Coatings to Inhibit Fungal Growth on Sliced Fruits". J. Food Technol., 7, p.17-22 (2004).

  3. Bae K. P., and Moon M.K., "Applications of Chitin and Its Derivatives in Biological Medicine" Int J Mol Sci11(12): 5152–5164( 2010)

  4. Hong T.,* Peng Z., Thomas L. K., Shannon J. R., Shenda M. B., William P. W. and Snezna R., "Antibacterial action of a novel functionalized chitosan-arginine against gram-negative bacteria" ActaBiomater. 6(7): 2562–2571. Jul; (2010)

  5. Helander, I.M., Nurmiaho-Lassila, E.-L., Ahvenainen, R., Rhoades, J., and Roller, S. "Chitosan disrupts the barrier properties of the outer membrane of Gram-negative" (2001)

  6. Kumar A.B., Varadaraj M.C., Gowda L.R. and R.N. Tharanathan.. "Characterization of chito-oligosaccharides prepared by chitosanolysis with the aid of papain and Pronase, and their bactericidal action against Bacillus cereus and Escherichia coli" Published as BJ Immediate Publication 2 June167–175( 2005).

  7. Kumar, M.N.V. "A review of chitin and chitosan applications" Reactive FunctPolym 46: 1–27(2000) .

  8. Kumar, A.B.V., Varadaraj, M.C., Gowda, L.R., and Tharanathan, R.N. "papain and pronase, and their bactericidal action against Bacillus cereus and Escherichia coli". Biochem J 391: 167–175 ( 2003 ).

  9. Liu, H., Du, Y.M., Wang, X.H., and Sun, L.P. "Chitosa kills bacteria through cell membrane n damage" Int J Food Microbiol 95: 147– 155(2004).

  10. Pochanavanich, P., and Suntornsuk, W. "Fungal chitosan production and its characterization" LettApplMicrobiol 35: 17–21(2002).

  11. Pochanavanich, P., and Suntornsuk, W. "Fungal chitosan production and its characterization" LettAppl Microbiol35: 17–21(2002).

  12. Raafat, D., von Bargen, K., Haas, A., and Sahl, H.-G. "Insights into the mode of action of chitosan as an antibacterial compound"Appl Environ Microbial 74: 3764–3773(2008).

  13. Savard, T., Beaulieu, C., Boucher, I., and Champagne, C.P. "Antimicrobial action of hydrolyzed chitosan against poilage yeasts and lactic acid bacteri of fermented a vegetables "Int J Food Prot 65: 828–833(2002).

  14. Singla, A.K., and Chawla, M. "Chitosan: some pharmaceutical and biological aspects – an update" J. Pharmacology 53: 1047–1067(2001).

  15. Torr, K.M., Chittenden, C., Franich, R.A., and Kreber, B "Advances in understanding. bioactivity of chitosan and chitosan oligomers against selected wood- inhabiting fungi " Holzforschung 59: 559–567(2005).