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Volume 6
Research & Reviews: Journal of Material Sciences
ISSN: 2321-6212
Advanced Materials 2018
September 04-06, 2018
September 04-06, 2018 | Zürich, Switzerland
21
st
International Conference on
Advanced Materials & Nanotechnology
Chitosan delaying human fibroblast senescence through down regulation of TGF-β signaling pathway
Tai-Horng Young
National Taiwan University, Taiwan
T
his study evaluated the effect of chitosan, poly vinyl alcohol (PVA) and poly (2-hydroxyethyl methacrylate) (pHEMA) on
delaying the human fibroblast senescence. Cells could form suspending multicellular spheroids on these biomaterials, but
only chitosan was capable of decreasing the SA β-gal activity and increasing the proliferation ability of senescent fibroblasts.
Therefore, in addition to the structure of multicellular spheroids, chitosan itself should play an important role in delaying
fibroblast senescence. The main difference of senescence related protein expressions for cells cultured on chitosan, PVA and
pHEMA occurred on the TGF-β signaling pathway. In addition to the intracellular TGF-β expression, the extracellular TGF-β
expression was also down regulated. Chitosan with cationic amino structure was assumed to bind with anionic TGF-β by
forming polyelectrolyte complexes. This assumption was demonstrated by directly adding chitosan into the medium to down
regulate the cell TGF-β expression and further to delay cell senescence, indicating TGF-β signaling pathway was involved in
the chitosan mediating fibroblast senescence process. Finally, the delaying cell senescence ability of chitosan increased with
increasing the amount of amino groups in chitosan and its ionization degree. In summary, these results provide important
information for considering the application of chitosan in the future cell therapy and regeneration medicine.
Figure:
The diagram of chitosan affecting senescent-related pathways.
Recent Publications
1. C H Chang, DMau-Hsu, K C Chen, CWWei, C Y Chiu and T H Young (2018) Evaluation of digital real-time PCR assay
as a molecular diagnostic tool for single-cell analysis. Scientific Reports 8(1):3432.
2. C W Tsai and T H Young (2016) CD44 expression trends of mesenchymal stem-derived cell, cancer cell and fibroblast
spheroids on chitosan-coated surfaces. Pure and Applied Chemistry 88(9):843-852.
3. CW Tsai, Y T Kao, I N Chiang, J HWang and T H Young (2015) Chitosan treatment delays the induction of senescence
in human foreskin fibroblast strains. PLOS One 10(10): e0140747.
4. Y H Chen, S H Chang, T J Wang, I J Wang and T H Young (2013) Cell fractionation on pH-responsive chitosan surface.
Biomaterials 34(4): 854-863.
5. P J Lou, M Y Chiu, C C Chou, B W Liao and T H Young (2010) The effect of poly (ethylene-co-vinyl alcohol) on
senescence-associated alterations of human dermal fibroblasts. Biomaterials 31(7):1568-1577.
Biography
Tai-Horng Young is currently a Professor at National Taiwan University (NTU) in Taipei, Taiwan. Since 2000, he was a Full Professor at the Institute of Biomedical
Engineering, National Taiwan University. He received several awards, including outstanding research award of National Science Council, and has been the Chair
Professor of National Taiwan University since 2007. From 2008 to 2011, he served as the Director of Institute of Biomedical Engineering, National Taiwan University.
He has been a member of many academic societies and has been selected as the President of Formosa Association of Regenerative Medicine (2012-2016).
thyoung@ntu.edu.twTai-Horng Young, Res. Rev. J Mat. Sci. 2018, Volume 6
DOI: 10.4172/2321-6212-C3-021