Cariappa KB*, Sathisha TG and Hamsa Veena
Department of Biochemistry, Sri Siddhartha Medical College, Tumkur. Karnataka, India.
Received: 10 February 2014 Accepted: 26 February 2014
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Smoking is one of the prime causative factors for the CHD. Smoking leads to altered serum lipid levels which in turn lead to CHD. 50 male smokers and age matched 50 male nonsmokers were recruited for study. Estimation of serum triglycerides and LDL cholesterol was done. Significantly elevated levels of serum triglycerides and LDL cholesterol (p<0.001) was observed in smokers in contrast to non smokers. Smoking inadvertently affects serum lipids thereby accentuating CHD risk.
LDL -Cholesterol, Triglycerides, Cigarette Smoking, CHD
Heart diseases like Atherosclerosis and Coronary diseases are caused by various types of smoking . Cigarette smoking is as important and independent risk factor for above mentioned disorders . This is further authenticated by the dose response relationship between the number of cigarettes smoked per day and cardiovascular morbidity and mortality .
For coronary heart disease, a major risk factor is cigarette smoking more than 10 numbers per day . Cigarette smoking and Hypercholesterolaemia are suggested along with other risk factors contributing as major ones for CHD. Nicotine is the major component of cigarette smoking. Nicotine causes increased secretion of catacholamines resulting in increased lipolysis . This leads to high levels of triglycerides, cholesterol and VLDL, and low levels of HDL [6,7].
The aim of the study was to elicit the variation in the blood lipid levels between rural smokers and non-smokers.
The current study consisted of 50 male smokers and 50 ages matched male non-smokers as controls. They were in the age group of 25-35 years hailing from the rural area around Sri Siddhartha medical college.Informed consent was taken from the voluntarily participants. Smokers were smoking more than 10 cigarettes per day since 5 years .Diabetics, hypertensives, obese individuals, alcoholics and persons on lipid lowering drugs were excluded.
Fasting venous blood 5 ml was taken from cubital fossa. Samples were centrifuged at 3600rpm for 6 minutes. Supernatant clear serum was extracted to estimate serum LDL cholesterol and Triglycerides. 
LDL–cholesterol was determined by direct immunoturbidmetric assay. Triglycerides were estimated by enzymatic method.
The results of smokers and controls non smokers are tabulated as follows.
Values are expressed as mean +/- SD
There is a statistically significant (P<0.001) increase in the serum triglycerides and LDL cholesterol of smokers when compared to controls in non smokers.
It is a well-known fact that nicotine is one of the important constituent of tobacco which has predominant influence on increasing lipid levels in blood. The cause of high levels of blood lipids is due to the catacholamines and adenyl cyclase axis induced tissue lipolysis. This is initiated by the stimulation of nicotine. Smoking leads to increase in oxidised LDL-cholesterol which in turn initiates atherosclerosis .
Our study reveals statistically significant (P<0.001) levels of serum triglycerides and LDL cholesterol in smokers in comparison to non smokers. Increased levels of serum triglycerides and LDL cholesterol I smokers is in agreement with previous reports [11-13]. IHD is related to fasting levels of triglycerides and cholesterol .
Cessation of smoking will lessen the risk of developing ischemic heart disease in rural population Further studies in a larger rural population is suggested to formulate policy on banning smoking for the benefit of mankind.
Our study establishes a significant relevance between smokers and non smokers. Elevated levels of serum LDL-Cholesterol and triglycerides in smokers predispose them to CHD in comparison to non smokers.