Perspective Article Open Access

A Short Note on Liquid Chromatography and its Types

Abstract

Liquid chromatography is a physical separation technology that distributes the components of a liquid mixture across two immiscible phases, stationary and mobile. Adsorption chromatography, partition chromatography, ionexchange chromatography, size-exclusion chromatography, and affinity chromatography are the five categories of LC practice. The Reverse-Phase (RP) mode of the partition chromatography technique, which uses a nonpolar (hydrophobic) stationary phase and a polar mobile phase, is the most extensively utilised of these. The stationary matrix is made by attaching long-chain alkyl groups (e.g., n-octadecyl or C18) to the external and internal surfaces of irregularly or spherically shaped 5 m diameter porous silica particles in common applications.

Lin Fuxian

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