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Bioestimation of Desloratadine and Montelukast Sodium in Spiked Human Plasma by High Performance Thin Layer Chromatography
High performance thin layer chromatographic method has been developed for the bioestimation of Desloratadine (DSLR) and Montelukast (MON) in their combined dosage form. Merck HPTLC aluminum plates of silica gel G60 F254 was used for separation of combined with 250 μm thickness using Ethanol: Methanol: Ammonia formate solution: Ammonia (9:1:0.5:0.5 v/v/v/v) as mobile phase. HPTLC separation of the both drugs were carried out and followed by densitometric measurement was performed in the absorbance mode at 287 nm. The drugs were resolved satisfactorily with Rf values of 0.19 ± 0.03 and 0.86 ± 0.03 for DSLR and MON, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification. The developed HPTLC method can be applied for the bio estimation of DSLR and MONT in bulk drug and drug formulation.