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Biosynthesis and Characterization of Selenium Nanoparticles Produced by Terrestrial Actinomycete Streptomyces microflavus Strain FSHJ31

Abstract

During last decades, study on the development of eco-friendly processes for the production of selenium nanoparticles (Se NPs) have received much attention due to hazardous effects of chemical compounds used for nanoparticle preparation. The present study was designed to screen actinomycete strains able to produce Se NPs. Among isolated bacterial strains, a terrestrial actinomycete strain which was tolerant to Se4+ ions (200 μg/ml of) was launched as Se NPs producer. Morphological and biochemical characteristics as well as 16S rDNA gene analysis of the selected strain introduced it as Streptomyces microflavus strain FSHJ31. The biologically synthesized Se NPs was then purified using n-Octyl alcohol/water extraction system and characterized by UV-vis spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray (EDX) and Fourier transform infrared spectroscopy (FTIR) techniques. Analysis of the particle size distribution pattern of biogenic Se NPs via laser light scattering method demonstrated the size range of 28–123 nm for Se NPs with the 48 nm NPs being the most frequent particles.

Hamid Forootanfara, Bijan Zareb,c, Homasadat Fasihi-Bamd, Sahar Amirpour-Rostamia, Atefe Ameria, Mojtaba Shakibaiee and Mohammad Torabi Namif

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