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Developing Rapid and Reliable Regeneration System Using Cotyledonary-Node Method in Indian Soybean Genotypes (Glycine Max L.)

Abstract

Regeneration through mature cotyledonary node has set rapid regeneration of plants directly from explants which is more time-saving and presented as an effective strategy so we have developed regeneration protocol through single shoot using cotyledonary node a rapid and efficient protocol for three Indian soybean cultivars. Two explants were collected from single cotyledonary node and cultured on medium containing N6-benzylaminopurine (BAP) for germination, BAP and indole-3-butyric acid (IBA) for shoot induction, Gibberellic acid (GA3) for shoot elongation and IBA for rooting of explants. The best combination of hormones for all genotypes were obtained as germination of seeds on half B5 medium supplemented with 1.0 mgl-1 of BAP, shoot induction on full B5 medium having BAP 1.0 mgl-1 and IBA 0.2 mgl-1 and shoot elongation on GA3 0.75 mgl-1 in full MS medium. Under these conditions, the plantlets could be raised within 40-45 days. It was observed that selection of proper medium for regeneration of soybean can overcome genotype associated problems. This regeneration system can be used for soybean transformation

Alkesh Hada, Amit Kumar Gupta, Theboral Jeevaraj, Markandan Manickavasagam, Andy Ganapathi, Monica Jolly , Archana Sachdev

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