e-ISSN:2320-1215 p-ISSN: 2322-0112

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Research Article Open Access

Development and Validation of Simultaneous Equation Method for the Estimation of Metformin amd Sitagliptin by UV Spectroscopy


A simple, sensitive, rapid, economic, UV Spectroscopic method was developed for the estimation of Sitagliptin-Metformin in Pure and Tablet dosage forms. The linearity for Metformin was found between 200-400 μ/ml and between 20-45 μ/ml for Sitagliptin. Metformin showed the maximum absorbance at 232nm & Sitagliptin at 266nm and Validation parameters like Precision, Accuracy, and System suitability parameters were determined and examined by applying validated parameters.

Sujani PV, SS Phanindra, P Anasuya, Y Padamanabha Reddy and N Devanna

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