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Rhizopogon luteolus and Ganoderma adspersum extracts inhibit invasion through the crosstalk antioxidant activity and apoptosis induced by pAKT/Rb

Abstract

Objective: Lung cancer is the primary cause of cancer-related deaths globally, and in NSCLC cancers, kinase protein B (AKT) is associated with pathways such as proliferative, invasion and apoptosis. In this study, the relationship of mushroom extracts with these pathways was investigated. Mushrooms have a long history of use in traditional medicine to treat a variety of illnesses, and scientists have been exploring the potential use of their antioxidant and anticancer properties in modern medicine, a topic to which this paper contributes. Materials and methods: Mushroom extracts were collected in 2022 and the other phases of the experiment were completed in 2023. We studied the lung cancer cell lines h1299, pc-3 and pc-14. Extracts of Rhizopogon luteolus and Ganoderma adspersum were applied to these to determine the IC50 values. Then, proliferative and invasion effects, apoptosis and antioxidant effects were investigated. Finally, Western blotting was performed to investigate the pathways of these effects. Results: Rhizopogon luteolus and Ganoderma adspersum extracts have anti-proliferative and anti-invasive effects on lung cancer cell lines and induce apoptosis, which increases the antioxidant effect. The mushroom extracts work through the p-AKT and Rb pathways. Conclusions: We anticipate that Rhizopogon luteolus and Ganoderma adspersum extracts will make effective additions to cancer treatment as agents that work to suppress lung cancer cells via the p-Akt and Rb pathways.

Aydın Demiray*, Ege Rıza Karagur, Gulsen Tel-Cayan, Onur Tokgun, Hakan Akca, Mehmet Emin Duru

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