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Research Article Open Access

Identification of Amino Acid and Proteolytic Activity on Protein by Pineapple (Ananas Comosus ) Enzyme Extract

Abstract

It is derived from the Spanish word pina meaning pine cone that was used in 1398. 300 years later it was called pineapple in order to identify the fruit individually. Scientifically the pineapple is known as Ananas comosus belongs to the family Bromeliaceae. It is the leading edible member of the family Bromeliaceae. Pineapple pulp contains different nutrients, and pineapple peel is known to have antioxidants helpful in warding off free radicals. Sulfhydryl proteolytic enzymes are the difconstituents of bromelain. It has been found that pineapple is very good digestive enzyme, so we have to find out that this enzyme protein having proteolytic activity or not and we have to identify that what are the different type of protein and amino-acid present in that compound because enzyme is a protein, what are the different composition of protein we have to identify. After performing all the identification test of amino acids, we found following amino acids present in the pineapple: Cysteine, methionine, histidine and tyrosine. And by performing the proteolytic activity on gelatin and albumin by the UV Visible spectroscopy we can see that the pineapple enzyme can break the proteins. If this herbal formulation prepared into drug it is beneficial for the human kind.

Sarkar S, Mandal S, Sen D, Das N, Mondal T, Paul A, Chakrabarty S, Barik B B

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