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Isolation and Molecular Identification of a Serratia spp. from Suspected Neonatal Sepsis in Intensive Care Unit (ICU) of Basra Province, Iraq

Abstract

The genus Serratia , a member of Enterobacteriaceae family, plays an important role in nosocomial infections. It is responsible for numerous hospital outbreaks occurring in immunodefficient patients,particularly newborns and patients in intensive care unit (ICU). The current study included the collection of one hundred and sixty blood samples from neonatal patients who are suspected of infection by late onset sepsis in the neonatal intensive care unit (ICUs) of Maternity and childhood hospital in Basra province . All samples were tested by using blood cultures to isolation bacteria and molecular identification the positive blood cultures . Result of this study showed 8(5%) samples were found with positive blood cultures while 152(95%) given negative blood cultures. after identification by using the biochemical test,all (8) bacterial isolates were identified as Serratia spp. In the second part of this study the 16SrDNA nucleotides sequencing data results for eight alignments of bacterial isolates were showed (5) isolates No1,3,4,7 and 8 similarity the isolates that have accession number in genbank (KT741023.1,KT260868.1,KJ130057.1,KT260868 and KJ877667.1) respectively as Serratia marcescens , while (3) isolates No2,5and 6 similarity the isolates that have accession number in genbank (KC191827.1, FJ811864.1and KC191827.1) respectively as Serratia liquefaciens.

Saad S Mahdi

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