Perspective Article Open Access

Methodological Approaches for the Detection and Characterization of Drug-Resistant Bacteria

Abstract

The rise of drug-resistant bacteria represents a critical challenge to global health, necessitating robust and standardized methodologies for detection, characterization, and monitoring. Accurate identification of resistant organisms is essential for guiding clinical treatment, informing infection control strategies, and supporting surveillance systems. This methods-focused article outlines the key laboratory and molecular techniques used to detect and analyze drugresistant bacteria. It discusses conventional culture-based methods, antimicrobial susceptibility testing, molecular diagnostics, whole-genome sequencing, and emerging technologies such as metagenomics and artificial intelligenceassisted platforms. Additionally, the article highlights sample collection, quality control, and data interpretation considerations. Standardization, reproducibility, and integration of advanced technologies are emphasized as crucial components for improving diagnostic accuracy and addressing antimicrobial resistance effectively.

David Kim

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