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Research Article Open Access

Preparation and Characterization of Nanostructured Lipid Carrier (NLC) and Nanoemulsion Containing Vitamin D for Enrichment of Dairy Products


Nanostructured Lipid Carriers (NLC) and nanoemulsions are carriers to improve bioavailability, stability and solubility of bioactive compounds. The main objective of this paper was to develop NLC and nanoemulsion for the enrichment of dairy products of vitamin D. NLC containing Precirol and nanoemulsion containing vegetables oil was prepared and characterized for polydispersity index, particle size, zeta potential, particle shape, crystal properties, stability, encapsulation efficiency and releasing. NLC showed sizes in the range of 123.4 to 210.6 nm and nanoemulsion 137.6 to 171.6 nm. Optimum NLC and nanoemulsion formulation was selected for other analysis (such as morphological study, encapsulation efficiency, thermal analysis and release study. Scanning and transmission electron microscopy revealed that particles had approximately spherical shape. In gastric simulated solution (pH=1.2) 9.3% of vitamin D from NLC and 29.6% from nanoemulsion released which indicated that NLC and nanoemulsion can protect vitamin D against acidic conditions.

Zeinab Jafarifar*

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