Production and Optimization of Polyhydroxyalkanoate from Oleaginous Bacteria Bacillus Sp. ISTC1
The present effort involved isolation and screening of bacterial strain Bacillus sp. ISTC1 for production of PHA and optimization of selected process parameters for enhanced production of PHA and biomass. Screening of selected bacterial strain for PHA production was based on Nile red staining, fluorescence microscopic visualization, spectrofluorometric measurement of Nile red fluorescence of the bacterial culture having 0.5% (w/v) glucose as carbon source. The presence of 3-hydroxyvalerate revealed by GC–MS served as a confirmatory analysis of PHA accumulation. Detection of characteristic functional group by FT-IR further confirmed the production of PHA by the bacterium. Response Surface Methodology (RSM) was used for optimization of pH, time duration and carbon source concentrations for an increased PHA production. On equating the optimized condition with the pre-optimized one, there was almost a 60% increase in the production of PHA. Therefore the finding thus established the production of PHA by Bacillus sp. ISTC1.
Manish Kumar, Juhi Gupta and Indu Shekhar Thakur