Research Article Open Access

Superior Efficacy of Egyptian Anticandidal Drugs Via combined therapy with Syzygium aromaticum Extract

Abstract

Candida spp. cause a very serious health problematic issue called candidiasis which affects many patients especially females. Egyptian pharmaceutical market EPM is full of many antifungal drugs with variable degrees of efficacy for many reasons. Thereafter, this ongoing study aimed to overcome the resistant problem emerged by Candida spp. by combination with clove extract. Isolation of Candida spp. from different areas overall Egypt including females, males and children (1-92 years-old), A huge number of yeast isolates were recovered; hence, CHROM agar medium CAMC was used for Candida differentiation as well as preliminary identification. Susceptibility test was performed using both of antifungal discs and antifungal drugs which had been already established in Egyptian pharmaceutical market in addition to some plant extracts using different solvents. Combination between plant extract and antifungal drugs was evaluated, cytotoxicity By MTT assay and finally prediction of subcomponents by GC-MS. The activity of each drug was determined, and the most resistant isolates were reported, chloroformmethanol 2:1 (v/v) and clove were succeeded together to exhibit antifungal activity. Mixture of clove extract and antifungal drugs was evaluated and fortunately, the In vitro results were hopeful due to the superior activities of this mixture which surpassed both clove extract and the drugs individually. The cytotoxic test exhibited perceptible cytotoxicity that is matching the topical usage of antifungal drugs which were applied superficially not to be administrated orally or via injection, and finally, GC-MS displayed presence of sitosterol which reflects the antifungal property

Xiyan Wang, Hongxi Guo

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