Research Article Open Access

The Regulatory Role of Casein Glycomacropeptide (CGMP) in the Fecal Flora of Mice with Ulcerative Colitis

Abstract

Objective: To explore the regulatory role of Casein glycomacropeptide (CGMP) in the fecal flora of mice with Ulcerative colitis (UC). Methods: The BALB/c mice were divided into a normal control group, a model group, CGMP groups with low doses (5 mg/kg·d), middle doses (50 mg/kg·d) and high doses (500 mg/kg·d), and a Sulfasalazine (SASP) group. Except the normal control group, all mice were treated with Oxazolone (OXZ) by perfusion to induce the UC model. In contrast, the mice in the normal control group and the model group were treated with an equal volume of saline for 7 consecutive days, which the changes in the fecal flora of the mouse intestines were evaluated by Fluorescence in situ hybridization (FISH) and DNA staining. Conclusion: CGMP can rectify the structural imbalances in the colon fecal flora in mice with UC and can improve the proportion of dominant flora in the intestine. CGMP at a dose of 50 mg/kg·d can significantly reduce the number of Enterobacteriaceae and Enterococci and promote the proliferation of Bifidobacteria, Lactobacilli, Bacteroides and Clostridium coccoides in the intestine.

Zhu Ming, Xueji Liu, Yali Yan, Guangchang Pang, Qingsen Chen

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