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Anatase TiO2 nanosheets were prepared by wet chemical method in the presence of Tripropylamine as capping ligand. The obtained samples were characterized by Powder X-ray diffraction, Raman spectroscopy, Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, Field emission scanning electron microscopy, Transmission electron microscopy and high-resolution transmission electron microscopy. The growth mechanism for the formation of TiO2 nanosheet has been investigated. The photovoltaic properties of anatase TiO2 nanosheets were investigated by assembling the TiO2 nanosheets as photoanodes in dye-sensitized solar cells (DSSCs). Experimentally, it shows that the anatase phase of TiO2 nanosheet films have conversion efficiency of 3.25%.