Research Article Open Access

Application of Rhizobacteria for Induction of Systemic Resistance in Brassica Campestris L. Against Alternaria Leaf Spot Disease Caused by Alternaria Brassicae

Abstract

Alternaria leaf spot disease is an important disorder causing serious yield loss of Brassica spp. The current study was designed to evaluate the role of rhizobacteria in management of Alternaria leaf spot disease in Brassica campestris. For this purpose B. campestris seedlings were treated with fifteen rhizobacterial strains under greenhouse and field conditions. The effect of rhizobacteria on quantity of defence related biochemicals such as PAL, PPO, PO and total phenols was examined. The Bacillus megaterium ZMR-6 and Pseudomonas fluorescens RB4 treated plants were least affected by the pathogen under greenhouse condition. Moreover, B. megaterium ZMR-6 and P. fluorescens RB4 exhibited significantly higher amount of defence related biochemicals. The growth promoting traits of bacteria B. megaterium ZMR-6 and P. fluorescens RB4 showed these were capable to exhibit phosphate solubilization, siderophore production and auxin synthesis. The B. megaterium ZMR-6 exhibited ACCD activity but P. fluorescens RB4 devoid this character. The application of peat moss based carrier material of B. megaterium ZMR-6 and P. fluorescens RB4 under field conditions showed promising results for disease management and agro-economic aspects of B. campestris. The current research revealed that B. megaterium ZMR-6 and P. fluorescens RB4 induce systemic resistance against Alternaria leaf spot and enhance yield in B. campestris.

Nasim Ahmad Yasin, Waheed Ullah Khan, Waheed Akram, Aqeel Ahmad, Yaseen Ashraf, Aamir Ali

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