Review Article Open Access
Measurement of Breast Cancer by PCR method
The aim of this study was optimization of Real Time PCR condition as breast cancer is one of the most common malignancies among women in various countries. HER2 overexpression occurs in 20-30% of breast cancers. HER2 gene encodes 185kDa transmembrane glycoprotein with 1255 amino acids. This active product triggers downstream intracellular signaling pathways inducing cell proliferation and cell survival. These activities can be done in an uncontrolled manner in the cases which HER2 expression undergoes up-regulation. In this study, total RNA was extracted from fresh tissue samples, first strand of total cDNA was synthesized and in the following steps, Real Time PCR was performed to be optimized.
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