Research Article Open Access

Method Development and Validation for Glucosamine and Chondroitin Sulphate in Soflet Form by RP-HPLC.

Abstract

To develop a simple, economic, accurate reverse phase isocratic RP-HPLC simultaneous estimation method for the Glucosamine(500mg) and Chondroitin Sulphate(400mg) in Soflet dosage form. Extended-C18 (YMC pack ODS-250 x 4.6 mm, packed with 5 micron) in an isocratic mode with mobile phase Octane sulphonic acid in water : Acetonitrile : triethylamine (90.65 : 8.96 : 0.381) was used and adjusted the pH 4.0 with ortho phosphoric acid. The flow rate was 1.0ml/ min and effluent was monitored at 195 nm. The retention times were 1.72 min and 1.353 min for Glucosamine and Chondroitin Sulphate respectively. The linearity range was found to be 80 to 120%. The proposed method was validated. The reports was expressed that the proposed method was found to be simple, precise, accurate and rapid for determination of Glucosamine(500mg) and Chondroitin Sulphate(400mg) in Soflet dosage form.

Parthiban Nagarajan, Sathis Kumar Dinakaran, Somsubhra Ghosh, Harani Avasarala, Maheswaran Nallayya, and David Banji

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