Engineering protein interfaces yields ferritin disassembly and reassembly under benign experimental conditions

21^st International Conference on Advanced Materials & Nanotechnology

September 04-06, 2018 | Zurich, Switzerland

Hai Chen

China Agricultural University, China

ScientificTracks Abstracts: Res. Rev. J Mat. Sci

DOI: 10.4172/2321-6212-C3-020

Abstract

Ferritin is a class of naturally occurring iron storage protein; it usually consists of 24 subunits that form a hollow protein shell with high symmetry. Recently, scientists have subverted these nature functions and used reversibly self-assembled property of apoferritin cage controlled by pH for the encapsulation and delivery of bioactive nutrients or anticancer drug. In all these cases, the ferritin cages shield their cargo from the influence of external conditions and provide a controlled microenvironment. However, since ferritin disassociation generally needs extreme acidic condition (pH≤2), this strategy is limited to the structures of bioactive compounds that are unstable at such low pH. Here, we engineered protein interfaces to yield ferritin nano cages which disassemble at pH 4.0 and reassemble at pH 7.5. During this process, bioactive molecules can be encapsulated within protein cavity. Thus, this engineered protein has the potential to be exploited as an alternative nano carrier for pH-sensitive bioactive compounds or drugs. Recent Publications 1. Chen H, Zhang S, Xu C and Zhao G (2016) Engineering protein interfaces yields ferritin disassembly and reassembly under benign experimental conditions. Chemical Communications 52(46):7402-7405. 2. Zang J, Chen H, Zhao G, Wang F and Ren F (2017) Ferritin cage for encapsulation and delivery of bioactive nutrients: From structure, property to applications. Critical reviews in food science and nutrition 57(17):3673-3683. 3. Zhang S, Zang J, Chen H, Li M, Xu C and Zhao G (2017) The size flexibility of ferritin nano cage opens a new way to prepare nanomaterials. Small 13(37):1701045- 1701051. 4. Zhang S, Zang J, Wang W, Chen H, Zhang X, Wang F and Zhao G (2016) Conversion of the native 24‐mer ferritin Nanocage into its non‐native 16‐mer analogue by insertion of extra amino acid residues. Angewandte Chemie 55(52):16064-16070. 5. Zhang S, Zang J, Zhang X, Chen H, Mikami B and Zhao G (2016) Silent amino acid residues at key subunit interfaces regulate the geometry of protein nanocages. ACS nano 10(11):10382-10388.

Biography

Hai Chen is a PhD student in College of Food Science and Nutritional Engineering at China Agricultural University, working on Engineering and Application of Protein Nanostructure, especially Ferritin Nano Cages.

E-mail: chenhai2509@163.com

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