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Pathology 2018
Research & Reviews: Journal of Medical and Health Sciences
ISSN: 2319-9865
Page 68
October 08-09, 2018
Edinburgh, Scotland
17
th
International Conference on
Pathology & Cancer
Epidemiology
Objective:
Several phenotype variants of factor B (FB) can be
identified in human serum. We have previously shown that
there are differences in concentration and activity of various
phenotypes FB. It was also observed that during serum storage
a slow conversion rate between FB phenotypes is accelerated at
37℃. The purpose of this work is to determine the difference of
conversion rate between different FB phenotype variants.
Methodology:
107 serum samples of healthy individuals
were investigated. Electrophoresis with immuno fixation was
used for phenotyping. Activity of FB was determined using
the kinetic, nephelometric test with FB deficient serum. RID
and electro immuno assay were used for determination of FB
concentration. C3 component conversion was investigated by
cross- electrophoresis.
Results:
In analyzed samples 60 SS, 43 FS, 3 FF and one F1S
phenotype was found. Three serum samples of each phenotype
were stored at 37ºC. After 24 h, 3 and 7 days in each sample FB
concentration and activity and complement alternative pathway
activity were determined. During conversion it was noticed that
complement activity was lost on the 7th day, while FB activity
remained unchanged. Using cross-electrophoresis we found that
on 7th day, C3 component was completely converted leading to
the loss of complement hemolytic activity.
Conclusion:
Phenotype FF had the fastest conversion rate,
FS phenotype slower and the slowest conversion rate had SS
phenotype. The differences were most obvious on the third day
of conversion. Various FB phenotypes had no influence on C3
component conversion.
gagabeg@gmail.comSpontaneous
in vitro
conversion of various phenotype forms of
factor B
Dragana Begovic
and
Dragisa Misovic
Clinical Health Center, Belgrade, Serbia
RRJMHS 2018
Volume: 7