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Objective: The aim of this study was to investigate and evaluate the self-reported oral health knowledge, attitude and practice among university students in Myanmar. It was also aimed to provide the data essentials to planning, implementation, evaluation of the services for the prevention, control, treatment and to set up priorities among those services. Furthermore, identifying the need for oral hygiene, awareness programs, policy and strategies for oral health literacy. Methods: The descriptive cross-sectional study was done among a total number of 611 university students which were selected by using the simple random sampling method from universities around Myanmar. Data were collected by self-administered, online-based structured questionnaire which was distributed through representatives of each including university. Results: Among 611 participants aged from 16 to 35 years who completed the evaluations, 37.8% and 62.2% were males and females respectively. Result shows that 100% of the participants performed tooth brushing. Regarding the frequency of tooth brushing, the majority of the respondents (79%) performed tooth brushing twice a day, while 13.8% performed once a day and 7.2% performed more than twice a day. 16.2% of the respondents had abnormal oral habits. 52.5% did not know meaning of the dental plaque. Moreover, 44 % of the respondents did not aware the reasons of gum bleeding.