Periodontal Treatment: Flap Surgery vs. Laser
		
                    
                                Vahini Pravalika K1*, Baanu Prakash G2
1Department of Pharmacology, Vivekananda College of Pharmacy, India
2Department of Medicinal Chemistry, MNR College of Pharmacy, India
  - *Corresponding Author:
- Vahini Pravalika K
 Masters in Pharmacology
 Vivekananda College of Pharmacy
 Hyderabad- 500018, Telangana, India
 Tel: +7799225867
 E-mail: pravalika.vh@gmail.com
Received Date: 12/10/2016; Accepted Date: 17/10/2016; Published Date: 24/10/2016
                Visit for more related articles at Research & Reviews: Journal of Dental Sciences
         
        
        
                Abstract
        Constant periodontitis is characterized as irritation of the gingiva stretching out into the adjoining connection mechanical assembly. The sickness is described by loss of clinical connection because of demolition of the periodontal tendon and loss of the adjoining supporting bone. Non-surgical periodontal strategies like scaling and root arranging took after by periodontal surgical systems are done to re-establish the periodontal wellbeing and capacity. Late headways like lasers, microsurgery and other treatment helps have set a point of reference in the field of periodontics. Lasers have changed various ventures, and oral consideration is no exemption. Despite the fact that laser periodontal treatment is still in its outset – and not yet considered a demonstrated technique for treatment.
                Keywords
Periodontitis, Oral hygiene, Microscopic organisms,
  Plaque
 Periodontal Disease Condition
Periodontal or gum ailment is a contamination of the supporting tissues and bone that hold your teeth set up. The condition influences for the most part grown-ups in their 30s and 40s [1-5].
• Poor maintenance of oral hygiene
• Chronic illnesses, such as diabetes
• High dose specific medications
• Smoking and chewing tobacco
Regular treatment for periodontal malady is scaling and root planning (SRP), which is a non-surgical technique for cleaning the surfaces of the teeth and their root which might be presented because of gum subsidence. This evacuates tartar, plaque and microscopic organisms from the gum encompassing the root, and advances the solid recovery of the gum tissue [5-10]. In periodontal laser treatment, the supplier utilizes a dental laser to get to and expel the aroused gum tissue from around the foundation of the tooth. At the point when the contaminated tissue is expelled and the root is uncovered, the root scaling starts [11,12]. This includes scratching off the analytics and plaque developed underneath the gum line and around the root. The dental practitioner then smoothens the root with instruments to expel any harsh detects that may pull in microorganisms and cause future diseases. The zone between the gum and the root can then recover amid the mending procedure [13-21].
Advantages and Disadvantages
• No general soporific is required, as is once in a while required for different types of dental surgery.
• Lasers can focus on the unhealthy ranges unequivocally and precisely.
• Bleeding, torment and swelling are constrained on the grounds that periodontal laser treatment is less intrusive than standard surgery.
• Recovery and recuperating times are shorter.
Constant periodontitis is a standout amongst the most common oral conditions the world over. Pathogenic plaque small scale greenery, host invulnerable reactions, and natural variables assume a noteworthy etiologic part and cause both immediate and also have interceded tissue harm [21-30]. Elimination or change of these variables is the essential part of treatment, which captures or controls the illness procedure. What's more, the point is to recover the tissues and re-establish capacity with strategies, which are unsurprising and accomplish long haul benefits [31-40].
Different strategies for improving recovery, for example, bio-mimetics, unions and obstruction layers have appeared to profit the treatment result, yet may expand the treatment costs, and extra intrusive methodology may not be adequate to the patient [41-43]. Laser innovation, particularly the diode laser is picking up prominence when all is said in done dental practice with potential advantages in an extensive variety of uses. Lasers have furnished us with a potential other option to at the same time expel the unhealthy delicate tissues, focus on the smaller scale
  living beings and in addition invigorate wound recuperating. A few lasers, for example, the carbon dioxide, Ho: YAG, Nd: YAG, diode, Er: YAG has been tentatively used for delicate tissue periodontal strategies. Laser use delivers less postoperative swelling, decreases aggravation and is likewise moderately effortless [44-58].
Complete mechanical debridement being the "highest quality level" of periodontal treatment, still does not dispose
  of the small scale living beings in the delicate tissue mass of the pocket, nor is finished resection of the sick tissues conceivable [59-63]. Extra delicate tissue curettage systems utilizing ultrasonic and different chemicals and in
  addition a few adjunctive privately conveyed specialists, for example, antimicrobials, sterile operators, mitigating operators, and host - regulating specialists have been assessed for improving the treatment result of ceaseless periodontitis with changing degrees of accomplishment. However, the consistency of these medications is not sure and antimicrobial medications may prompt the improvement of safe microbial strains [64-73].
A few clinical studies have bolstered the antibacterial impact of lasers in periodontal pockets. Some studies have even reported tissue recovery on histologic assessment taking after laser interceded periodontal treatment using the "laser helped new connection methodology." Furthermore, a few creators have reported improved results utilizing lasers to de-epithelize the inward coating of the fold in light of the guideline of guided tissue recovery [74-78].
Cost and Procedural Aspects
Laser gum surgery costs about 8,000 USD, contingent upon the seriousness of your gum condition. The expense is practically identical to conventional fold surgery and ought to be secured under most dental protection arranged.
  The noteworthy target of fold surgery is to dispose of or diminish the pocket itself. To get to it, a fold like cut is made
  in the gum tissue. This permits unhealthy tissue to be expelled from inside the pocket, and gives access to the teeth's root surfaces for an exhaustive cleaning, which kills hurtful plaque and math (tartar). A short time later, the
  "fold" is shut, fixing the zone. This starts the recuperating procedure, which happens quickly [79-82].
Fold surgery is commonly done under nearby anesthesia, here and there joined by oral hostile to nervousness solutions; on the other hand, it might be performed under intravenous cognizant sedation. After anesthesia has produced results, a little entry point is made to isolate the gums from the teeth. The external gum tissue is tenderly collapsed back to offer access to the roots and the supporting tendon and bone tissue [83-86].
Next, the aroused gum tissue can be expelled, and the tooth roots can be cleaned; if necessary, the territory may likewise be treated with anti-infection agents or different solutions. Bone deformities can be repaired with
  joining material, and appropriate recovery of the periodontal tendon can be empowered by physical (hindrance films) and concoction (development components) strategies. At long last, the cut is shut and the method is finished [87,88]. Performed by an accomplished hand, cutting edge fold surgery has a superb reputation and offers settled advantages. It's regularly the treatment of decision for calming periodontal ailment and keeping up your oral wellbeing-and save your teeth.
Relief from all the below aspects can be experienced slowly or immediately after the periodontal flap or laser surgery:
• Gum Bleeding
• Numbness or feeling of Sensitivity
• Gum/Nerve Swelling
• Bruising due to treatment
• Sutures as per procedure
• Surgical Dressings needs to be maintained regularly
• Dental Discomfort
• Staining or side effects due to certain Medications used
Laser-assisted new attachment procedure (LANAP) is a surgical treatment for the treatment of periodontitis, expected to work through recovery as opposed to resection [89-91]. One of the primary advantages for utilizing dental lasers is the capacity to decisively communicate and, at times, Expel very nearly a couple cell layers at once. Erbium lasers can have some selectivity in evacuating unhealthy tooth structure, since carious sores have much higher water content than solid tissue. Contemplates have indicated other favorable circumstances over ordinary rapid hand piece communication on the tooth surface, for example, the disposal of smaller scale breaks and a reported bringing down of pulpal temperature as the arrangement continues. Bony tissue expulsion and molding can  likewise continue effortlessly with reported speedier recuperating. In addition, it has been shown that the lased veneer has a decent potential for reinforced reclamations the length of they are in this manner carved with corrosive [92-94].
Lasers likewise permit the clinician to diminish the measure of microscopic organisms and different pathogens in the surgical field and in the cavity readiness; and, on account of delicate tissue strategies, accomplish great hemostasis with the lessened requirement for sutures and surgical pressing. A few original copies call
  attention to that post agent scar arrangement is minimized; since the laser entry point is more expansive and unpredictable than that of a surgical tool, the mending tissue better mixes with the encompassing structures. Periodontal unhealthy tissue can be sanitized and detoxified [95-98].
Lasers can effectively and securely be utilized on an extensive variety of the populace, for example, youngsters and pregnant ladies not at all like some endorsed and/or sulcularly conveyed drugs. Dissimilar to those
  prescriptions, the patient won't encounter unfavourably susceptible responses, bacterial resistance, or untoward symptoms when the laser is utilized. With great control of seeping, there is significantly enhanced perception of the surgical field, and numerous laser strategies can be performed with less injectable anaesthesia. In those
  circumstances, extra treatment may have the capacity to be performed on the same arrangement. Moreover, starting postoperative uneasiness and swelling are diminished as a result of the fixing of nerves and lymphatic’s [99].
There are a few burdens to the present instruments. Without further ado accessible dental lasers just discharge vitality from the tip of the conveyance framework; and, in that sense, they are all "end cutting," which as a rule means an alteration of the expert's clinical procedure. In spite of the fact that they are helpful for caries evacuation and tooth arrangement, the Erbium group of lasers can't evacuate gold and vitreous porcelain, and has just a little collaboration with amalgam. Obviously, that reality is likewise favorable position while treating a repetitive carious injury nearby a lacquer or crown, for instance, since there will be no association with the
  therapeutic material. Nonetheless, most composite rebuilding efforts can be removed [98]. At times, the conveyance framework can be more awkward than an air rotor or electric hand piece, and availability to the treatment territory could be restricted. The clinician should deliberately watch and screen the rate of tissue expulsion to anticipate
  overheating and parallel warm harm. For veneer evacuation, the laser is not as quick as a turning burr, in spite of
  the fact that it can be more traditionalists by not evacuating as much solid tooth structure. The underlying venture for a few gadgets must be considered, and in addition required supplies and support. The instruments extend in size from a soft cover novel to a huge dental truck, so coordination of space can turn into a consideration. All units work at line voltage and the Erbium lasers require an extra air supply [100].
Preparing and proceeding with training are crucial, and most producer’s give great backing. The Foundation
  of Laser Dentistry offers a Standard Proficiency Course that instructs the expert at a sensible starting competency. A few diaries and a couple of course books are promptly accessible for depictions of clinical systems and research.
References
  - Borisenko  LG. Oral health and dental care of old adults in Byelorussia. Oral  Health Dent Manag. 2003;2:155.
- Jadidfard  MP, et al. Social insurance for dental care in Iran:A developing scheme for a  developing country. Oral Health Dent Manag. 2012;11:480.
- Achtari  MD, et al. Dental care throughout pregnancy:what a  dentist must know. Oral Health Dent Manag. 2012;11:477.
- Somya  K and Surendra L. Nanotechnology (nanohydroxyapatite crystals):recent  advancement in treatment of dentinal hypersensitivity. J Interdiscipl Med  Dent Sci. 2015;3:181.
- Pravin  M, et al. A critical analysis of dentation and dental care in ayurveda. J  HomeopAyurv Med. 2014;3:175.
- Reis  PF, et al. Dental care clothing:an investigation the presence of bacterial  contamination by public health professionals in southern Brazil. J Food  Process Technol. 2015;6:407.
- Ranta  TRV, et al. Mandibular advancement device therapy in obstructive sleep  apnea and snoring in community dental care:two-year follow-up study on  self-reported sleep quality, side effects, and compliance. J Sleep  DisordTher. 2014;3:180.
- Hatami  B, et al. Dental students' perceived barriers in geriatric dental care active  involvement. Oral Health Dent Manag. 2014;13:650.
- Sullivan  AL, et al. Exploring obstacles for dental care among the SCI population. Int  J Phys Med Rehabil. 2013;1:154.
- Alshehri A. Social  and behavioral determinants of early childhood caries in the aseer region of Saudi  Arabia. Pediatr Dent Care. 2016;1:114.
- Godzieba A, et  al. An analysis of the variability of hemodynamic parameters in patients  with ischaemic heart disease undergoing dental surgery:a randomized clinical  trial. Dental Sciences. 2016;4:100-105.
- Bhargava ASK, et al.  Relation between water and salivary fluoride levels among children residing in  communities having different naturally occuring water fluoride levels in Andhra  Pradesh, India. Biol Med. 2013.
- Ghimire N. Oral  Health? An integral part of general health. J Mass Communicat Journalism.  2013;3:e138.
- Boid A, et al. A  comparative evaluation of systemic azithromycin and ornidazole-ofloxacin  combination as an adjunct to scaling and root planning in the treatment of  chronic generalised periodontitis. Dentistry. 2015;5:309.
- Nanditha S, et  al. A judicious treatment approach for the management of localized  aggressive periodontitis:a case report. J Interdiscipl Med Dent Sci.  2015;3:174.
- Alwithanani N, et al. Periodontal  treatment improves prostate symptoms and lowers serum psa in men with high PSA  and chronic periodontitis. Dentistry. 2015;5:284.
- Pushparani DS. Low  serum zinc and increased acid phosphatase activity in type 2 diabetes mellitus  with periodontitis subjects. BiochemPharmacol (Los Angel). 2015;4:162.
- Evers F, et al. Periodontitis,  an often-overlooked reservoir for bacteria, in a patient with decubital ulcer. ClinMicrobiol.  2015;4:189.
- Policicchio P. Diabetes  and periodontitis: a two way relationship. Dental Sciences. 2014.
- Kazi MM, et al. An  approach to the microbiological diagnosis of chronic periodontitis:an overview. Dental  Sciences. 2014.
- Gokhale SA, et  al. A study to evaluate the relationship between Il-1β and Tnf-Α levels  in Gcf in non-smoker, previous or current smoker female patients with chronic  periodontitis. Dentistry. 2014;4:270.
- Bulgin D. Chronic  periodontitis and systemic health. Oral Hyg Health. 2014;2:e109.
- Zaghloul MZ. Human  herpes viruses in patients with chronic periodontitis and aggressive  periodontitis. Air Water Borne Diseases. 2014;e131.
- Mummolo S, et al. Oral  antiseptic and periodontitis:a clinical and microbiological study. Oral  Health Dent Manag. 2014;13:657.
- Pillai H, et al. Primary  sinus carcinoma masquerading as destructive periodontitis:report of a case. Dentistry.  2014;4:251.
- Cayci E, et al. Peripheral  giant cell reparative granuloma of maxilla in a patient with aggressive  periodontitis. Oral Health Dent Manag. 2014;13:642.
- Fenol A, et al. Levels  of interleukin-10 in gingival crevicular fluid and its role in the initiation  and progression of gingivitis to periodontitis. Oral Hyg Health.  2014;2:135.
- Kakade SP and Thakkar  K. Inter-disciplinary approach ought to periodontitis and type 1 diabetes  mellitus in india. J Diabetes Metab. 2014;5:385.
- Herrmann JM and Meyle  J. White blood cell signaling and defense mechanisms in patients with  diabetes mellitus type 2 and periodontitis. J Clin Trials. 2014;4:171.
- Mokeem SA, et al. Influence  of smoking on clinical parameters and gingival crevicular fluid volume in  patients with chronic periodontitis. Oral Health Dent Manag. 2014;13:611.
- Iwai T and Umeda  M. Smoking, periodontitis and vascular disease-collaboration study with  dentists and vascular surgeons. J Interdiscipl Med Dent Sci. 2014;2:113.
- Zaghloul MZ. The  contribution of anaerobic bacteria that causes periodontitis in the  pathogenesis of chronic obstructive pulmonary disease (COPD). Air Water  Borne Diseases. 2014;3:e128.
- Dababneh RH and Bissada  NF. Syndromes that include both Palmoplantarkeratoderma and severe  periodontitis:A Review. Dentistry. 2014;4:186.
- Robledo JG. Apical  periodontitis treatment:surgical-non surgical? Oral Hyg Health. 2013;1:e104.
- Goldberg MB, et al. Two  case reports on refractory periodontitis:systemic implications and a potential  new therapeutic strategy. J Interdiscipl Med Dent Sci. 2013;1:101.
- Bey A, et al.  Periodontitis:a significant risk factor for preterm low birth weight (PTLBW)  babies. Biology and medicine.
- Irina SA, et  al. Generalised pre-puberal periodontitis:literature review and case  presentation. Oral Health Dent Manag. 2007;6:318.
- Maita E, et al. Oral  hygiene instruction to arrest periodontitis in adolescents. Oral Health  Dent Manag. 2004;3:167.
- Leroy R, et al. Methodological  issues in epidemiological studies of periodontitis:how can it be improved? Oral  Health Dent Manag. 2010;9:415.
- In vitro antifungal  susceptibility of Candida albicans isolates from patients with chronic  periodontitis and diabetes. ClinMicrobiol 2013;2:103.
- Nwhator SO and Adedigba  MA. Is periodontitis the missing link? A metaethnographic review of  glycemic control measures by Nigerian diabetologists. Dentistry.  2012;2:121.
- Eskandari A, et al. Comparing  the effect of one stage full mouth disinfection versus quadrant-wise scaling  and root planning on clinical parameters and salivary inflammatory biomarkers  in chronic periodontitis patients. Dentistry. 2012;2:118.
- Michaela T, et al. Periodontitis  and preterm low birth weight: is there any association? ReprodSyst Sex  Disord 2012;S2:001.
- Argento MA, et  al. Flapless technique for periodontal bone grafts in treatment of severe  periodontitis. Presentation and long-term retrospective study. JMBT. 2010;2:107-110.
- Kim HD, et al. Omic  paradigms enhance interface between periodontitis pathogenesis and human health. J  Nutr Food Sci. 2016;6:506.
- Zacarias JMV, et  al. Letter to the editor concerning: the role of human leukocyte antigen  typing in Libyan patients with chronic periodontitis. J Infect Dis Ther.  2016;4:279.
- Edson da Silva, et al. Diabetes  mellitus and periodontitis:molecular interrelationships. J immuno Biol.  2016;1:e102.
- Rathod V, et al. Study  to evaluate the effects of periodontitis and type ii diabetes on serum and  salivary superoxide dismutase levels. Dental Sciences. 2015.
- Daeki AO, et al. The  role of human leukocyte antigen typing in Libyan patients with chronic  periodontitis. J Infect Dis Ther. 2015;3:5.
- Barot VJ and Chandran  S (2015) Statistical study on serum CRP levels in aggressive &  chronic periodontitis: pre & post non-surgical periodontal therapy. Anaplastology.
- Taani DS. Dental health  of 13-14-year-old Jordanian school children and its relationship with  socio-economic status. Int J Paediatr Dent. 1996;6:183-186.
- Genco RJ. Current view  of risk factors for periodontal diseases. J Periodontol. 1996;67:1041-1049.
- Offenbacher S, et al.  Periodontal infection as a possible risk factor for preterm low birth weight. J  Periodontol. 1996;67:1103-1113.
- Maurya DK, et al. Role  of triphala in the management of peridontal disease. AncSci Life.  1997;17:120-127.
- Arendorf TM, et al.  Oral manifestations of HIV infection in 600 South African patients. J Oral  Pathol Med. 1998;27:176-179.
- Fontana G, et al. An immunological  evaluation of type II diabetic patients with periodontal disease. J Diabetes  Complications. 1999;13:23-30.
- Barczyk MP, et al.  Brain abscess as a rare complication in a hemodialysed patient. Med SciMonit.  2001;7:1329-1333.
- Rasmussen L, et al. Characterization  of bone resorbing activity in gingival crevicular fluid from patients with  periodontitis. J ClinPeriodontol. 2000;27:41-52.
- Brook I. Microbiology  and management of periodontal infections. Gen Dent. 2003;51:424-428.
- Seymour RA. Dentistry  and the medically compromised patient. Surgeon. 2003;1:207-214.
- Persson GR, et al.  Influence of interleukin-1 gene polymorphism on the outcome of supportive  periodontal therapy explored by a multi-factorial periodontal risk assessment  model (PRA). Oral Health Prev Dent. 2003;1:17-27.
- Takashiba S and  Naruishi K. Gene polymorphisms in periodontal health and disease. Periodontol  2000. 2006;40:94-106.
- Hullah E, et al.  Self-reported oral hygiene habits, dental attendance and attitudes to dentistry  during pregnancy in a sample of immigrant women in North London. Arch Gynecol  Obstet. 2008;277:405-409.
- Gajewska MC, et al.  Influence of smoking on incidence and progression of periodontal disease.  Review of the literature. PrzeglLek. 2008;65:249-251. 
- Cakić S. Gingival crevicular  fluid in the diagnosis of periodontal and systemic diseases. SrpArhCelokLek.  2009;137:298-303.
- Grant WB and Boucher  BJ. Are Hill's criteria for causality satisfied for vitamin D and periodontal  disease? Dermatoendocrinol. 2010;2:30-36.
- Ramirez JH, et al. Why  must physicians know about oral diseases? Teach Learn Med. 2010;22:148-155.
- Wahlström M, et al.  Clinical follow-up of unilateral, fixed dental prosthesis on maxillary  implants. Clin Oral Implants Res. 2010;21:1294-1300. 
- Zulfiqar M, et al. Oral  Fusobacteriumnucleatum subsp. polymorphum binds to human salivary α-amylase.  Mol Oral Microbiol. 2013;28:425-434.
- Ahmed U and Tanwir F.  Association of periodontal pathogenesis and cardiovascular diseases:a  literature review. Oral Health Prev Dent. 2015;13:21-27.
- Novacek G, et al.  Dental and periodontal disease in patients with cirrhosis--role of etiology of  liver disease. J Hepatol. 1995;22:576-582.
- Mengel R, et al.  Periodontal health of the population in eastern Germany (former GDR). J  ClinPeriodontol. 1993;20:752-755.
- Pommereau DV, et al.  Insulin-dependent diabetes and periodontal disease in young patients. Ann  Pediatr (Paris) 1991;38:235-239.
- Suzuki JB. Diagnosis  and classification of the periodontal diseases. Dent Clin North Am.  1988;32:195-216.
- Wolff LF, et al. Salt  and peroxide compared with conventional oral hygiene. II. Microbial results. J  Periodontol. 1987;58:301-307.
- Newman MG. Anaerobic  oral and dental infection. Rev Infect Dis. 1984;6:S107-S114.
- Wierzbicka M, et al.  Dental caries, periodontal diseases and the condition of mouth mucosa in  workers of the petrochemical industry. Med Pr. 1983;34:275-281.
- Maeda N. Anaerobic,  gram-positive, pleomorphic rods in human gingival crevice. Bull Tokyo Med Dent  Univ. 1980;27:63-70.
- Bjorvatn K, et al. Oral  aspects of osteopetrosis. Scand J Dent Res. 1979;87:245-252.
- Markkanen H, et al.  Evaluation of periodontal status in a Finnish industrial population. Community  Dent Oral Epidemiol. 1979;7:96-100.
- Merle JP.  Gingivectomies. Flap operations. Rev StomatolChirMaxillofac. 1979;80:12-15.
- Gordon HA, et al. Blood  flow, collagen components of oral tissue and salivary kallikrein in young to  senescent, germfree and conventional rats. A study on the etiologic factors of  periodontal disease. Gerontology. 1978;24:1-11.
- Ramfjord SP. Surgical  pocket therapy. Int Dent J. 1977;27:263-269.
- Melnick M, et al.  Tricho-dento-osseous syndrome:a scanning electron microscopic analysis. Clin  Genet. 1977;12:17-27.
- Graf E. Functional  analysis in orthodontics:diagnosis of mandibular positions and movements. SSO  SchweizMonatsschrZahnheilkd. 1977;87:245-256.
- Curilović Z and Renggli  HH. Peridontal conditions in a group of Tibetans. SSO  SchweizMonatsschrZahnheilkd. 1976;86:1135-1143.
- Baker JJ, et al.  Importance of Actinomyces and certain gram-negative anaerobic organisms in the  transformation of lymphocytes from patients with periodontal disease. Infect  Immun. 1976;13:1363-1368.
- Penzer V. Letter to the  editors: Re: A new concept of peridontal disease by Walter Drum, J Periodontal  46:504, 1975. J Periodontol. 1976;47:104.
- Ketterl W. Pharmacology  and periodontology. DtschZahnarztl Z. 1975;30:368-371.
- Longhurst P. Peridontal  disease in childhood. Proc Br Paedod Soc. 1974;4:17-20.
- Ruben MP, et al. The  histopathology of peridontal disease in children. J Periodontol.  1971;42:473-484.
- Tanzer JM and Hageage  GJ. Polyphosphate inhibition of growth of plaques formed by streptococci and  diphtheroids implicated in oral disease. Infect Immun. 1970;1:604-606.
- Pezzoli M and Borio PS.  The treatment of acute pulpal and peridontal disease by means of the  para-apical infiltration of corticosteroids]. Minerva Stomatol.  1969;18:549-556.
- Powell RN and Alexander  AG. The treatment of peridontal disease. 5. Elimination of the  lesion-subgingival scaling and curettage. Br Dent J. 1966;120:10-11.
- Chawla TN, et al. Blood  changes in peridontal disease. J All India Dent Assoc. 1965;37:224-227.
- Fullmer HM. A critique  of normal connective tissues of the periodontium and some alterations with  peridontal disease. J Dent Res. 1962;41:223-234.
- Baer PN and Newton WL.  Studies on peridontal disease in the mouse. The germ-free mouse and its  conventional control. Oral Surg Oral Med Oral Pathol. 1960;13:1134-1144.
- Chawla TN and Mehta FS.  Review on epidemiology of peridontal disease. J All India Dent Assoc.  1960;32:121-129.
- Patrone A and Colli JC.  Natural amino acids in the diet & the treatment of periodontal diseases.  Sem Med. 1958;27:1000-1003.
- Gebhardt  H. Criticism of customary peridontal therapy with reference to the hypotension  theory and its practical results. ZahnarztlRundsch. 1954;20:698-701.