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Combination of Hairy Roots Explants and 6-Benzylaminopurine (BA) as an Alternative Improvement for In-Vitro Plant Regeneration of Capsicum spp

Abstract

Capsicum annuum and Capsicum frutescens belong to Solanaceae family are known for tremendous economic importance as crops and medicine. However, in-vitro regeneration of Capsicum spp. can be quite challenging. Therefore, in this research study, the uses of hairy roots explants with the 6-Benzylaminopurine (BA) was found highly effective to increase chances during in-vitro regeneration of both Capsicum annuum and Capsicum frutescens. The result had showed that, cotyledon explants that were co-cultured with selective Agrobacterium rhizogenes strain which were ATTC 15834 for C. frutescens and ATTC 43056 for C. annuum with BA hormones in five different concentrations (0.25, 0.5, 1.0, 1.5, 2.0 mg/L) for two months had promising outcomes for morphogenesis. From the study, the optimum concentrations of BA in C. frutescens (1.0 mg/L) and C. annuum (1.5 mg/L) had reduced the shoot induction days with lacked of callus formation and necrosis. Even though, the different concentrations of BA able to enhance the direct morphogenesis of both Capsicum spp. however there were only slightly morphology differences had been noted in both Capsicum species. As a conclusion, the study of using both hairy roots induced cotyledon explants with BA at optimum concentration had shown high potential in regenerating this species by producing leaf, stem and root as well as reducing possibility of recalcitrant.

Nursuria Md Setamam and Norrizah Jaafar Sidik

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