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Research Article Open Access

Development and Validation of a RP-HPLC Method for Simultaneous Estimation of Diacerein and Aceclofenac in Pharmaceutical Dosage Form

Abstract

A new simple, accurate, precise and reproducible RP-HPLC method has been developed for the simultaneous estimation of Diacerein and Aceclofenac in pharmaceutical dosage form using C18 column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of Acetonitrile and Water in the ratio of 55:45 (v/v). The detection wavelength was carried out at 271 nm. The method was linear over the concentration range for Aceclofenac 50-75 μg/ml and for Diacerein 100-150 μg/ml. The recoveries of Aceclofenac and Diacerein were found to be in the range of 99.23-100.39% and 99.45-100.56% respectively. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form.

Narendra Nyola, Govinda Samy Jeyabalan, N Kalra, Gazala Parveen, and Suresh Choudhary

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