Formulation and Evaluation of Quercetin Nanoparticles using Low Molecular Chitosan by Qbd Approach
Objective: The aim of the study is the formulation and evaluation of Quercetin nanoparticles using low molecular weight chitosan by applying QbD approach.
Materials and methods: The Quercetin nanoparticles are prepared by ionic gelation technique, low molecular weight chitosan was used as the polymer. In this study 23 Factorial design (FD) with two center points was used for optimization. The independent variables were speed, time and cross linking agent to polymer ratio. The dependent variables were particle size and entrapment efficiency. The resulting data was fitted into Design Expert Software (Trial Version) and analyzed statistically using analysis of variance (ANOVA). The data was also subjected to response surface methodology to determine the influence of speed, time and cross linking agent to polymer ratio on responses. The physicochemical properties of the prepared Quercetin nanoparticles were characterized by Particle Size, Particle Size Distribution, Zeta Potential, Surface Morphology Drug encapsulation efficiency FT-infrared spectrophotometer, In-vitro release Study and Kinetic Study.
Results: The average particle size of optimized Quercetin nanoparticles was found to be 97.5 nm with entrapment efficiency of 50% and zeta potential of +24.7 mv. The drug release profile showed that there was a burst release of 30.7% in first 5 hrs and 93% was released in 36 hrs. In release kinetic study the release profile had a best fit with Higuchi model with R2 value of 0.995.
Conclusion: The optimized Quercetin nanoparticles can be used as effective drug in the targeting of cancer due to its size and drug release characteristics.
M. Anita Sharma, P.M.D Sameer, I.Nooral Alam, AN. Rajalakshmi