Research Article Open Access

Quantitation of Oleanolic Acid in Rat Plasma Using Modifier-assisted Differential Mobility Spectrometry Tandem Mass Spectrometry Coupled with Multiple Ion Monitoring

Abstract

Bioanalysis of oleanolic acid in rat plasma using Differential Mobility Spectrometry (DMS) tandem mass spectrometry combined with Multiple Ion Monitoring (MIM) method was developed and validated. Oleanolic acid, a kind of pentacyclic triterpenoid compound derived from plants, has various biological activities. It is difficult to determine using LC-MS/MS because it has poor Collision-Induced Dissociation (CID) efficiency when analyzing in Multiple Reaction Monitoring (MRM) mode. In order to increase the sensitivity and simplify the biological sample preparation, we explored a UPLC-DMS-MIM approach. Good accuracy and precision were achieved in the range 3-300 ng/mL with a run time of only 3 min. Isopropanol as organic modifier facilitated a good combination of sensitivity and separation. The method is a proof-of-principle report of the analysis of compounds that unable to generate stable and abundant fragment due to too much fragmentation or poor CID efficiency.

Jingkai Gu, Chunsu Liang, Chong Su, Yingze Liu, Tianming Ren

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