Commentary Open Access

Ultra-Performance Liquid Chromatography (UPLC): Revolutionizing Analytical Chemistry

Abstract

Ultra-performance liquid chromatography (UPLC) represents a significant advancement over traditional high-performance liquid chromatography (HPLC), offering higher resolution, faster analysis, and increased sensitivity. By utilizing sub-2 μm particle size columns and operating at higher pressures, UPLC achieves superior chromatographic efficiency, enabling the separation and quantification of complex mixtures with unprecedented accuracy. This technology has wideranging applications in pharmaceuticals, environmental monitoring, food analysis, and biochemical research. Coupled with sophisticated detectors such as UV-Vis, fluorescence, and mass spectrometry (MS), UPLC enhances impurity profiling, pharmacokinetics, quality control, and biomolecule analysis. This article reviews the principles, instrumentation, methodology, applications, challenges, and emerging trends of UPLC, emphasizing its transformative impact on analytical chemistry.

Isabella R. Thompson

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