e-ISSN No.:2581-3897

Epidemiological Investigation of Bovine Trypanosomosis and its Vector Apparent Densities in Yayo District Illuababora Zone, Western Oromia, Ethiopia

Kitila G1, Kebede B3*, Guta D2, Bekele F2, Wagari M2, Tilahun B2 and Jaleta D2

1Ethiopian Public Health Institute, Addis Ababa, Ethiopia

2Bedelle Regional Veterinary Laboratory Center, Bedelle, Ethiopia

3Veterinary Drug and Animal Feed Administration and Control Authority, Addis Ababa, Ethiopia

*Corresponding Author:
Kebede B
Veterinary Drug and Animal Feed Administration and Control Authority
Addis Ababa, Ethiopia
E-mail: kebede.bedaso@yahoo.com

Received Date: November 09 2016; Accepted Date: December 28 2016; Published Date: December 30 2016

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Abstract

The cross-sectional study was carried out from January to May, 2015 to assess prevalence of bovine trypanosomosis and apparent density of its vectors in Yayo district Iluababora zone of western Oromia. In current study 408 cattle were randomly selected for the study of bovine trypanosomosis prevalence in the district. Blood sample were collected from ear vein of cattle drawn into heparinized capillary tube then centrifuged and buffy coat techniques and thin blood smear were used to identify species of trypanosomes. This revealed that overall prevalence 7.4%, 95% CI: 5.1- 10.0% with the highest rate of infection of Trypanosoma congolense (66.7%) than Trypanosoma vivax 8(26.7%) and mixed infection 2(6.7%). Prevalence of trypanosomosis higher in female cattle 8%, 95% CI: 5.0- 11.6%, old cattle 25%, 95% CI: 0.0-62.5% and poor body condition score cattle 9.3%, 95% CI: 3.7-14.8% and they were insignificantly associated with the bovine trypanosomosis prevalence. Bovine trypanosomosis prevalence was higher in aneamic 26(7.54%), poor body condition score (10(9.26%), female 21(8.11%) and old cattle 28(7.49%) and all the risk factors are insignificantly associated with trypanosomosis prevalence. The mean PCV of the infected cattle, male cattle and medium body condition score cattle were 17.767%, 95% CI: 15.96-19.58%, 20.274%, 95% CI: 19.78-20.77%, 20.09%, 95% CI: 19.53-20.65% lower than their perspective, respectively. In study 45 monopyramidal traps used to assess apparent density of tsetse flies and other biting flies in the district were Glossina pallidipes and G.f. fuscipes caught with overall apparent density of 142.41 FTD and other biting flies Tabanus, Stomoxys and Heamatopota. This indicates that how much tsetse flies and trypanosomosis challenge the livelihood of farmers. Therefore, chemoprophylaxis and prophylactics techniques and vector control techniques should be implemented.

Keywords

Prevalence, risk factors, Bovine trypanosomosis, Apparent densities, Yayo district

Introduction

Bovine trypanosomosis is disease that results in scarcity of animal production and productivity in sub-Saharan African countries which include western and southwestern parts of Ethiopia. It covers over 10 millions of square kilometers of potentially productive land [1-2]. Vector borne trypanosomosis is excluding some 180,000–200,000 km2 of agriculturally suitable land in the west and southwestern parts of the country; leaving 14 million of cattle, equivalent number of goat’s and more than 7.5 million equines and 2.3 million of camels are at risk of contracting trypanosomosis [3].

Trypanosomosis is one of the diseases that are caused by flagellated protozoan parasites which belong to the genus Trypanosoma. Trypanosoma is a unicellular parasite found in the blood and other tissues of vertebrates including livestock, wild life and people [4]. The species of trypanosomes are known to exist in Ethiopia, which are pathogenic to cattle, are Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei. They are distributed mainly in tsetse belt region of the Ethiopia. However, T. vivax is also found in areas outside of the tsetse belt, where it can possibly be transmitted by mechanical vectors of biting flies [5-6].According to National Tsetse and Trypanosomosis Investigation and Control Center [2], tsetse transmitted animal trypanosomosis still remain as one of the largest cause of livestock production losses in Ethiopia. Trypanosomosis is characterisations are intermittent fever, anaemia, immunodepression, heartfailure, oedemas and wasting. Methods used to reduce trypanosomiasis and its effects are use of drugs for curative and preventive and use of trypanotolerant cattle (Muturu and N'Dama). Economic impacts developed are direct losses (loss of production, mortality and abortion), cost of control (cost of drugs, transportation cost to field and operators salaries) and indirect losses (loss of potential production) [4,7].

There are 22 different species of tsetse flies inhabit Africa. These species are arranged into three groups based on their male genitalia construction. Groups are morsitans, palpalis and fusca. Studies interest for tsetse distribution are emanated from firstly tsetse belts continue from one country to the next, across the national boundary, secondly an expansion of a tsetse belt in one country may cause the belt to come up to and cross an international boundary and thirdly knowing the continental distribution of tsetse species gives a greater understanding of what stops the fly from spreading further, and whether a spread beyond the present limits in a particular country is likely. Distribution of morsitans group is limited by climatic (cold winter and hot dry) conditions and scarcity of game animals as well it is the most widespread group. However, palpalis group limited to very humid areas and fusca group limited to thickly forested areas. Tsetses are able to find their hosts by their sense of smell and by sight. Tsetse fly importance is its ability of spreading trypanosomosis among domestic animals and man. For this reason, very large areas of Africa are without cattle which are prevented economic development these areas. Tsetse infested areas lie in the lowlands and also in the river valleys of Abay (Blue Nile), Baro, Akobo, Didessa, Ghibe, and Omo in Ethiopia. Five species of Glossina (G. morsitans submorsitans, G. pallidipes, G. tachinoides, G. fuscipes fuscipes and G. longipennis) have been recorded in Ethiopia [2,4,5].

In Yayo district there is a paucity of information on the trypanosomosis and tsetse flies. Therefore, the aim of this study is to determine prevalence of bovine trypanosomosis and distribution of tsetse flies.

Materials and Methods

Study area, population and sample size determination

The study was conducted from January to June, 2015 in Yayo district, Western Oromia, which is situated at 550 km West of Addis Ababa in Ilu Aba Bora Zone. The mean annual rainfall in Yayo district ranges from 1000 to 1500 mm. The annual temperature ranges from 15-31°C. The altitude of the area ranges from 1,000 to 2060 m above sea level (m.a.s.l). The Geba forest which is registered on the United Nations Educational, Scientific, and Cultural Organization (UNESCO) for its natural habitats is located in the study area. The area has a number of wild animals, such as African buffaloes, Bush pigs, warthog, bush buck, kudu, hippopotamus, crocodiles, hyena, antelopes and snakes which are claimed to serve as sources of food for the vector of trypanosomes. The cattle in the district are local breeds that are kept under traditional extensive husbandry systems with communal herding. Agriculture is the main livelihood of the society with mixed farming system and livestock play an integral role for agriculture. Currently, at the time of study the livestock populations of district were 60,000 cattle, 30,120 Sheep, 35,120 Goat, 5,232 Donkey, 4,693 Horse, 2,722 Mule and 80,000 Poultry. Sample size was determined using 95% confidence level, 50% expected prevalence and 0.05 desired absolute precision using the formula described by Thrusfield [8]. Therefore, a total of 408 cattle were randomly examined for bovine trypanosomosis to increase precision.

Study design and protocol

Yayo district was selected purposely based on the extent of the existing problems, the complaints of farmers and the level of medium to high tsetse challenge in the area from the report of the field veterinarian in the district. A cross-sectional study design was engaged and three peasant associations were selected based on the veterinary reports of the trypanosomosis and tsetse infestation in the district. Cattle body condition score was categorized as good, medium and poor and their age was categorized as young (<3 years old), adult (3 to 9 years old) and old (>9 years old) according to Nicholson and Butterworth [9].

Sample collection

Buffy coat technique was used immediately as samples collected for the determination of bovine trypanosomosis prevalence. Blood sample collection was performed by piercing the marginal ear vein with a sterile lancet and blood was drawn by a heparinized capillary tube. Then one end (the heparinized end) of capillary tubes were sealed with crystal sealant and centrifuged at 12,000 rpm for five minutes to separate the blood cells and to concentrate trypanosomes using centrifugal forces. Then the Packed Cell Volume (PCV) was determined by packed cell volume reader and recorded. The PCV value ≥25 and <25 were considered as non-aneamic and aneamic, respectively. The capillary tubes were then broken just below buffy coat using diamond pencil and expressed on microscopic slide and covered with a cover slip. It was examined under 40X objective of microscope to identify and detect the presence of the parasites [10]. Those positive were thin smeared to be stain by geimsa staining for further identification into species using their morphological characteristics under oil immersion microscope in laboratory.

Entomological survey

For the entomological survey a total of 45 monopyramidal baited traps were deployed in three PA’s of altitude ranges from 1237-1500 m a.s.l. to assess the apparent density, distributions and species of tsetse flies and other biting flies involved in transmission of trypanosomosis. All traps were baited with acetone, Octenol (1-3-Octane) and cow urine filled in separated bottles and labeled and deployed at an interval of 200-250 m. After 72 h of trap deployment, the cages were collected and captured flies were identified and sexed according to morphological characteristics, and counted. The tsetse flies were identified as species level and the other biting flies as the genus level. The apparent density was determined based on the mean catches of flies in traps deployed and expressed as the number of fly catch/trap/day [11].

Data management and analysis

Raw data were entered into a Microsoft Excel spreadsheet and descriptive statistics were used to summarize the data. The prevalence was calculated for all data as the number of infected individuals divided by the number of individuals examined and multiplied by 100. The association between the prevalence of trypanosome infection and risk factors were assessed by chisquare, whereas the student’s t-test was used to assess the difference in mean PCV between trypanosome positive, negative and overall examined animals. All statistical analyses were conducted using SPSS version 20.0 software. The test result was considered significant when the calculated p-value was less than 0.05. The apparent density of fly population was calculated by dividing the number of flies caught by the number of traps deployed and the number of days of deployment and expressed as fly/ trap/day (FTD).

Results

Trypanosomosis survey

In current study 408 cattle were randomly selected for the study of bovine trypanosomosis prevalence in the district. Blood sample collected from ear vein of cattle drawn into heparinized capillary tube then centrifuged and buffy coat techniques used to detect the trypanosomes and those positive were thin blood smeared to identify morphologically species of trypanosomes. This study revealed that overall prevalence 7.4%, 95% CI: 5.1-10.0% with the highest rate of infection of T. congolonse (66.7%) than T. vivax 8(26.7%) and mixed infection 2(6.7%) and none of T. brucei were recorded and trypanosomosis highly infested Witete peasant association (9.7%) than the rest Tables 1 and 2. Prevalence of trypanosomosis higher in female cattle 8%, 95% CI: 5.0-11.6% than male cattle 6%, 95% CI: 2.0-10.1%; old cattle 25%, 95% CI: 0.0-62.5% than adult cattle 7.5%, 95% CI: 5.1-10.2%; poor body condition score cattle 9.3%, 95% CI: 3.7-14.8% than good body condition score cattle 8%, 95% CI: 3.4-14.5% and medium body condition score 6.1%, 95% CI: 2.8-9.9%; Witete peasant association 9.7%, 95% CI: 4.9-15.1% than Gere peasant association 6.7%, 95% CI: 2.5-12.5% and Webo peasant association 5.6%, 95%CI: 2.1-9.2% and all of them were insignificantly associated with the bovine trypanosomosis prevalence Table 3.

PA’s Sample size Positive Prevalence[95%CI] Trypanosoma Species
        Mixed T.congolence T.vivax T.brucei
Webo 144 8 5.6[2.1, 9.2] 2(1.4%) 4(2.8%) 2(1.4%) 0
Gere 120 8 6.7[2.5,12.5] 0 5(4.2%) 3(2.5%) 0
Witete 144 14 9.7[4.9,15.1] 0 11(7.6%) 3(2.1%) 0
Over all 408 30 7.4[5.1,10.0] 2(0.5%) 20(4.9%) 8(2%) 0

Table 1. Prevalence of Trypanosoma species with regard to PA’s.

Trypanosomes species Prevalence
T.congolense 20(66.7%)
Mixed infection 2(6.7%)
T. vivax 8(26.7%)

Table 2. Proportions of trypanosomes species

Risk factor Sample size Positive Prevalence[95%CI] P-value
Sex Male 149 9 6[2.0,10.1] 0.643
  Female 259 21 8[5.0,11.6]
Age Young 26 0 0 0.162
  Adult 374 28 7.5[5.1,10.2]
Old 8 2 25[0.0,62.5]
BCS Good 88 7 8[3.4,14.5] 0.436
  Medium 212 13 6.1[2.8,9.9]
Poor 108 10 9.3[3.7,14.8]
PA’s Gere 120 8 6.7[2.5,12.5] 0.246
  Webo 144 8 5.6[2.1,9.2]
Witete 144 14 9.7[4.9,15.1]

Table 3. Prevalence of trypanosomosis based on risk factor

Bovine trypanosomosis prevalence was higher in aneamic 26(7.54%) than non-aneamic 4(6.3%) cattle; cattle of poor body condition score 10(9.26%) was harbored more infection of trypanosomosis than good 7(7.95%) and medium 13(6.13%) body condition scores cattle; female cattle 21(8.11%) was more exposed to trypanosomosis than male cattle 9(6.04%), Witete peasant association 14(9.72%)was with highest infection of trypanosomosis than Webo 8(5.56%) and Gere 8(6.67%) peasant association; old cattle 28(7.49%) ported more infection than adult 28(7.49%) and all the risk factors are insignificantly associated with trypanosomosis prevalence (Table 4).

Risk factors Sample size Trypanosomosis prevalence Total X2 P-value
T.congolense Mixed(T.congolense and T.vivax) T.vivax
PCV Aneamic 345 16(4.6%) 2(0.6%) 8(2.3%) 26(7.54%) 2.153a 0.541
  Non-aneamic 63 4(6.3%) 0(0.0%) 0(0.0%) 0(0.0%) - -
BCS Good 88 4(4.5%) 1(1.1%) 2(2.3%) 7(7.95%) 5.889a 0.436
  Medium 212 7(3.3%) 1(0.5%) 5(2.4%) 13(6.13%) - -
Poor 108 9(8.3%) 0(0.0%) 1(0.9%) 10(9.26%) - -
Sex Female 259 13(5.0%) 2(0.8%) 6(2.3%) 21(8.11%) 1.672a 0.643
  Male 149 7(4.7%) 0(0.0%) 2(1.3%) 9(6.04%) - -
PA Gere 120 5(4.2%) 0(0.0%) 3(2.5%) 8(6.67%) 7.898a 0.246
  Webo 144 4(2.8%) 2(1.4%) 2(1.4%) 8(5.56%) - -
Witete 144 11(7.6%) 0(0.0%) 3(2.1%) 14(9.72%) - -
Age Adult 374 18(4.8%) 2(0.5%) 8(2.1%) 28(7.49%) 9.203a 0.162
  Old 8 2(2.5%) 0(0.0%) 0(0.0%) 2(25%) - -
  Young 26 0(0.0%) 0(0.0%) 0(0.0%) 0(0.0%) - -

Table 4. Association of trypanosomosis prevalence with risk factors.

Heamatological survey

The mean PCV of the infected cattle 17.767%, 95% CI: 15.96-19.58% lower than non-infected cattle 20.542%, 95% CI: 20.14-20.95%; male cattle 20.274%, 95% CI: 19.78-20.77% slightly lower than female 20.450%, 95% CI:19.76-21.14%; medium body condition score cattle 20.09%, 95% CI: 19.53-20.65% lower than good and poor body condition score cattle 20.51%, 95% CI: 19.59-21.43% and 20.68%, 95% CI: 19.96-21.41%, respectively (Table 5).

Risk factors Sample size Mean PCV 95%CI Std.dev. df P-value t-value
Trypanosomes infection
Infected 30 17.767 [15.96,19.58] 4.847 29 0.00 20.076
Non-infected 378 20.542 [20.14,20.95] 3.982 377 - 100.299
Sex
Male 259 20.274 [19.78,20.77] 4.012 258 0.00 81.319
Female 149 20.450 [19.76,21.14] 4.285 148 - 58.261
Body Condition scores
Poor 108 20.685 [19.96,21.41] 3.825 107 0.00 56.204
Medium 212 20.090 [19.53,20.65] 4.156 211 - 70.374
Good 88 20.511 [19.59,21.43] 4.334 87 - 44.399

Table 5. Association of PCV with the factors.

Entomological survey

This study incorporated 45 monopyramidal traps to assess apparent density of tsetse flies and other biting flies in the district. Species of Tsetse flies caught in the study areas were G. pallidipes and G.f. fuscipes with overall apparent density of 142.41 FTD. Other biting flies Tabanus, Stomoxys and Heamatopota were trapped during the study period. All monopyramidal traps were trapped within the range of 1237 m 1500 m altitude above sea level. Webo peasant association with the range of altitude 1237 m 1257 m were mostly infested with tsetse flies than other two peasant associations (Table 6).

PA’s Altitude No. of trap No. of days G.pallidipes G.f.fuscipes Total FTD Tabanus Stomoxys Heamatopota
Webo 1237-1257m 15 3 56,533 96     3 216 95
    56,629 1258.42  
Gere 1372-1500m 15 3 100 92     1 114 20
    192 4.26667  
Witete 1300-1498m 15 3 566 289     2 204 119
    855 19  
District   45 9 57,199 477 57,676 142.41 6 534 234

Table 6. Apparent densities of tsetse flies and other biting flies caught in three peasant associations

Discussion

The current study reveals that overall prevalence 7.4% in the study area. Similar findings ranges from 6.7% to 8.7% were reported in Dale Wabera and Dale sedi districts of Kellem Wollega zone, in southwestern Ethiopia and Guto Gida district of East Wollega zone [12-14]. However, prevalence ranges from 13.6% to 16.9% higher than the present finding were reported in Humbo district of southern Ethiopia, Chora district of Ilu Aba Bora zone and Sayonole district of western Oromia [15-17]. Eventhough, from Dale Wabera district of Kellem Wollega zone reported lower prevalence (2.86%) of trypanosomosis [18]. These differences were due to ecological differences and seasonal variations of study areas [19-20].

Species of trypanosomes predominant in the study area was T. congolonse (66.7%) followed by T. vivax 8(26.7%). This study agrees to previous report [12,14-18] that their prevalence ranges from 53.3% to 88.40%. Unlike to this finding T. vivax predominant in Cameroon and Nigeria [21-22]. This difference occurred because of geographical factors and G. pallidipes and G. m. sub-morsitans are efficiently transmits T. congolense than T. vivax in Eastern Africa. In addition, the predominance of T. congolense infection in cattle may be due to the high number of serodams of cattle as compared to T. vivax and development of better immune response to T. vivax by the infected animal [11].

The prevalence of trypanosomosis was higher in the female cattle 8%, 95% CI: 5.0-11.6%, old 25%, 95% CI: 0.0-62.5%, cattle of poor body condition score 9.3%, 95%CI: 3.70-14.8% and Witete peasant association 9.7%, 95% CI: 4.9-15.1% than rest of factors and insignificantly associated (P>0.05) to the prevalence of trypanosomosis. Similar with earlier report of Takile et al. [14] in Guto Gida district female (8.37%), Poor (19.67%) and old (9.21%), [16] in Chora district female (9.2%) and poor (5.2%) [17], in Sayonole district poor (19.7%) and [12] in Dale sedi and Wabera Poor (15%). In contrary to this, [18] and [12] determined male cattle harbor more infection than female cattle in Dale Wabera district male cattle (3.64%) and Dale sedi and Wabera district male cattle (10.7%), respectively. This could be due to female cattle were kept for purpose of rearing or giving offspring and milking for a long period of time and in numbers than male cattle used for ploughing purpose that lead in the continuous exposure of tsetse flies infestation. Old cattle stayed in the areas for long time that increases their exposure to the tsetse flies, but poor body condition score cattle deprived immunity to fight against infection of trypanosomosis.

Mean of packed cell volume (PCV) varies among risk factors. Trypanosomes infected cattle mean PCV 17.767%, 95% CI: 15.96-19.58% lower than non-infected cattle mean PCV 20.542%, 95% CI: 20.14-20. 95%. This could be due to aneamic nature of trypanosomosis and malnutrition. Mean PCV of male 20.274%, 95% CI: 19.78-20.77% lower than female cattle mean PCV 20.450%, 95% CI: 19.76-21.14%; medium body condition score PCV 20.090%, 95% CI: 19.53-20.65% lower than poor and good body condition score cattle 20.685%, 95% CI; 19.96-21.41% and 20.511%, 95% CI: 19.59-21.43%, respectively and all risk factors statistically associated (P<0.05) with PCV. Analogous to the previous report of [14] in Guto Gida (20.23%) district, [15] in Humbo (20.2%) district, [16] in Chora (19.02%) district, [12] in Sayonole (20.86%) district, [12] in Dale Wabera (20.9%) and Dale sedi (20.3%) districts and [23] in western Oromia (22.88%). Difference in aneamia status in trypanosome infected cattle was probably due to discrepancy in ecology [20], nutritional status [24], animal breed [25], season of the study [19], tsetse control and trypanocidal drug utilization level [26] and/or diagnostic method employed [27-29].

Monopyramidal type of traps 45 in number was deployed in three peasant associations of Yayo district. These traps were trapped G. pallidipes and G.f. fuscipes with their overall apparent density of 142.41 flies/traps/days and other biting flies Tabanus, Stomoxys and Heamatopota within the range of 1237–1500 m altitude above sea levels. Hence, G. pallidipes was predominantly with the apparent density 141.23 flies/traps/days in the district. Other study reported apparent density in Dale Wabera and Dale sedi (4.8 flies/traps/days) districts [12], Sayonole (13.01 flies/trap/days) district [17] and Chora (2.63 flies/traps/days) district [16] which is lower than the current study. Hence, in Abaya district apparent density of G. pallidipes 0.5 flies/traps/days was the predominant in the district, but this apparent density is lower than the current study G. pallidipes apparent density. Generally, this indicates that higher rate of tsetse flies infestation in the district which is more responsible for the trypanosomosis transmission.

Conclusion and Recommendation

Current study revealed that highest apparent density of tsetse flies that transmit trypanosomosis infection to cattle which severely harm production and productivity of livestock in the district. In the study area T. congolense and G. pallidipes were predominantly occurred in the study area. Hence, the apparent density of tsetse flies in the district was abundant than other areas with similar geography. Female, old and poor cattle were incredible infected by trypanosomes species and those infected, medium body condition score and male cattle possessed a lower mean packed cell volume. Based on this the following recommendations are forwarded:

a. Tsetse flies control techniques should be implemented.

b. Chemotherapeutics and prophylactics techniques should be undertaken.

References