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Cerium oxide nanoparticles have unique catalytic properties which make it a promising regenerative and a great free radical scavenger especially in the biomedical applications. Cerium oxide catalytic properties are affected by the relative fraction (%) of Ce3+ which in turn depends on the particle size. In the preset work, the catalytic properties of cerium oxide have been improved by increasing the relative fraction (%) of Ce3+. In order to reach this goal cerium oxide has been prepared in the nano-scale and the effect of treating these prepared nanoparticles by ionizing radiation of each cold plasma and gamma on the relative fraction (%) of Ce3+ has been studied.