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Approach Antimicrobial, Antioxidant and Anti-Inflammatory Activity of Hydroxychavicol on Cancer Cells

Jayashree Padhy* and K Aruna Lakshmi

Department of Biotechnology, GITAM University, GIT, Visakhapatnam, Andhra Pradesh, India

Corresponding Author:
Jayashree Padhy
M.Tech, Department of Biotechnology
Arthur Cotton Bhavan, GIT, GITAM
Rushikonda, Visakhapatnam-530045
Andhra Pradesh, India

Received Date: 03/08/2016; Accepted Date: 23/08/2016; Published Date: 28/08/2016

Visit for more related articles at Research & Reviews: Journal of Pharmacognosy and Phytochemistry


Hydroxychavicol an chloroform extract is a fluid concentrate of Piper beetle leaves demonstrated inhibitory movement against oral pit pathogens. The motivation behind it is to uncover the conceivable impact of this plant in the improvement of remedially dynamic home grown medications. Piper betle Linn., generally known as the beetle vine is a vital therapeutic and recreational plant in Southeast Asia. Flute player betle is celebrated as evergreen and enduring plant that God outlined and have given the state of his own heart. Betle vines are one of the very explored plants and their phytochemical ponders demonstrate that Piper betle contains a wide assortment of naturally dynamic intensifies whose focus relies on upon the assortment of the plant, season and atmosphere


Anticancer, Antioxidant, Apoptosis, Cervical cancer, HeLa cell line, Piperaceae


Hydroxychavicol (HCH), a phenolic compound of Piper betle leaves has hostile to mutagenic and against cancer-causing movement. Antimicrobial, cell reinforcement and mitigating properties were additionally ascribed to HCH. Late writing recommends that HCH can possibly dispose of prostate tumor cells. Concentrates likewise recommended apoptosis of oral carcinoma cells by HCH through acceptance of responsive oxygen species (ROS) [1-5]. Hydroxychavicol is a to a great degree intense xanthine oxidase inhibitor with an IC50 esteem 0.0167 μM, Its more strong than allopurinol.

Hydroxychavicol (HCH), a constituent of Piper betle leaf has been accounted for to apply hostile to leukemic action through enlistment of receptive oxygen species (ROS) [6]. The point of the study is to enhance the oxidative anxiety –induced endless myeloid leukemic (CML) cell demise by consolidating glutathione union inhibitor, buthionine sulfoximine (BSO) with HCH and examining the hidden system [7-10].

Alcoholic concentrate of Piper betle (Piper betle L.) leaves was as of late found to initiate apoptosis of CML cells communicating wild sort and transformed Bcr-Abl with imatinib resistance phenotype. Hydroxychavicol (HCH), a constituent of the alcoholic concentrate of Piper betle leaves, was assessed for hostile to CML action [11].

We have as of late shown that hydroxychavicol is a noteworthy constituent and the most dynamic biophenolic of Piper betel leaves with critical antiproliferative movement in the small scale molar reach. Thus we introduce the configuration, union and assessment of fifteen novel hydroxychavicol analogs with changing antiproliferative exercises in disease cell lines from two agent tissue sorts, to be specific, the prostate and cervix that show exceptionally promising results contrasted with the guardian mixes. Our long range objective is to build up a structure-movement guided relationship to increase unthinking bits of knowledge into novel atomic focuses of this class of bioactive particles for balanced medication improvement [12-15]. Cytotoxicity-guided experimentation on these novel analogs yielded the accompanying basic variables as the key movement controllers: 1) not at all like the hydroxyl substituent at position-4, the position-3 hydroxyl is fundamental for upgraded action 2) acetoxyl gatherings are superfluous for action as confirmed before by others 3) allylic twofold securities at 2'C-3'C serve to decidedly impact antiproliferative action 4) since a long time ago immersed side chains at 1'- position adversely direct antiproliferative action and 5) moving position-4 with a benzyl bunch emphatically affected the natural action
profile. Most amphiphilic mixes indicated moderate to great restorative potential obviously on the premise of therapeutic science standards [16-25].

Materials and Methods

The leaves of P. betle were separated with solvents of fluctuating polarities (water, methanol, ethyl acetic acid derivation and hexane) and their phenolic and flavonoid substance were resolved utilizing colorimetric tests. Phenolic organization was portrayed utilizing HPLC [26-30]. Cancer prevention agent exercises were measured utilizing
FRAP, DPPH, superoxide anion, nitric oxide and hyroxyl radical searching tests [31].

Developed leaves of high caliber were gathered from sound wines developed under characteristic conditions without presentation to pesticides and concoction composts. The leaves gathered were completely washed with refined water and smear dried in the research center. Ethanolic concentrate of the leaves was readied utilizing a
Soxhlet extractor [32-38]. Organic exercises of the concentrates were dissected utilizing MTT examine and cancer prevention agent protein (catalase, superoxide dismutase, glutathione peroxidase) measures in HeLa cell lines [39].

Cervical malignancy is the second most regular growth and in addition one of driving reason for tumor related demise for ladies around the world. As to that issue, center of this paper will be on prevalently utilized Piperaceae individuals including Piper betle L, Piper cf delicate Benth, Piper umbellatum L, Piper aduncum L, Piper pellucidum L [41-45]. This exploration was directed to illustrate the cancer prevention agent, anticancer and apoptosis actuating exercises of Piperaceae concentrates on cervical growth cells, specifically HeLa cell line [46].

All Piperaceae extricates have high anticancer action; longer hatching increment anticancer movement. P.betle separate has the most elevated cancer prevention agent property [47-52].

Hydroxychavicol (HCH), a phenolic compound of Piper betle leaves has been appeared to have against mutagenic and hostile to cancer-causing action. HCH has antimicrobial, cell reinforcement and mitigating properties. Late
concentrates additionally recommend apoptosis of oral (KB) carcinoma cells by HCH through acceptance of
receptive oxygen species (ROS). None of the past studies propose any systems downstream of ROS for HCHinstigated apoptosis [53-57].


The anticancer action was controlled by repressing the expansion of cells. Apoptosis prompting was dictated by restraining multiplication cells and by SubG1 stream cytometry [58]. The cancer prevention agent action is controlled by utilizing superoxide dismutase esteem and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical rummaging action [59-62].


The most elevated anticancer movement at 24 h hatching was found for P. pellucidum extricate (IC50: 2.85 μg/ml); The anticancer action at 48 h hatching was more than at 24 h for all concentrates [63-70]. The most noteworthy apoptotic action was found for P.betle (12.5 μg/ml) at both 24 and 48 h hatching. The most astounding cell reinforcement movement was additionally spoken to by P.betle extricate [73-80].

Flute player betle leaf has a critical anticancer and antibacterial action against wide range of small scale living beings (gram postivie microorganisms dn gram negative microscopic organisms). Locally accessible and effortlessly developed [81-85]. The antibacterial action of hydroxychavicol against E.coli, Shigella dysentrie, Salmonella typhi, S.aureus and S.pyogenes are accounted for interestingly. No past report on the antibacterial movement of these species could be found in the writing [86,87]. These microbial investigations of hydroxychavicol demonstrated the most encouraging antimicrobial properties showing the potential for the revelation of new novel medications from plants [88-92]. Further phytochemical studies are required to decide the sorts of dynamic mixes in charge of the antibacterial action of the flautist betle and to improvement of new plans are required. This plant could serve as helpful hotspots for new antimicrobial operators [93-95].