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Research Article Open Access

Analytical Method Development and Validation of Azelnipidine and Telmisartan by RP HPLC Method

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Azelnipidine and Telmisartan in tablet dosage form. Chromatogram was run through standard symmetry C18 (4.6 x 150 mm, 5 mm). Mobile phase containing Buffer 0.01 N KH2PO: Acetonitrile taken in the ratio 45:55% v/v was pumped through column at a flow rate of 1 ml/min. Optimized wavelength selected was 290 nm. Retention time of Azelnipidine and Telmisartan were found to be 2.131 min and 2.593 min. %RSD of the Azelnipidine and Telmisartan were and found to be 0.8 and 0.6 respectively. %Recovery was obtained as 99.59% and 99.94% for Azelnipidine and Telmisartan respectively. LOD, LOQ values obtained from regression equations of Azelnipidine and Telmisartan were 0.86, 2.60 and 0.09, 0.29 respectively. Regression equation of Azelnidipine is y=12533 x +10387 and y=9061 x +183.8 of Telmisartan. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Vangallu Spandana*, Siddartha

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