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Review Article Open Access



Bacteria present great importance in the clinic, where the mechanisms to combat these pathogens appear more and more to find a substance with low cost, good pharmacological properties and low toxicity. It can be mentioned that the main microbiological methodologies found in the literature for the detection of bacterial and fungal activity can be of three types: bio autographic, dilution and diffusion. Generally the techniques used are disk diffusion, cavity diffusion, agar paste, agar dilution and broth dilution. These assays are quantitative methods in which the effects can be classified. Experiments as an in vitro experimental model are the results of competition. The different types according to the method employed, such as diffusion assays in the medium, are capable of influencing the oxidation of compounds that favor the development of bacterial membrane. Objective: to gather the main methodologies applied in the antibacterial test and those that help to corroborate with the antimicrobial research, where comparisons will be made between methodologies applied by the authors, but with the intention of providing the execution protocol for each of the cited trials. The materials were collected in the main databases, using articles, books, technical manuals and international regulations, all in the most current contexts. Conclusion: the research gathered seven methodologies applicable directly to the antibacterial tests, followed by five tests that corroborate with the antibacterial results for the development of a possible pharmaceutical alternative, such as the experimental toxicity test with Drosophila megalogaster; Photodynamic Therapy (PDT) and cytoprotection against Microbial Heavy Metal Models.

Antonio Linkoln Alves Borges Leal*

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