Commentary Open Access

High-Performance Thin Layer Chromatography (HPTLC): Principles, Methodology, Applications, and Advances

Abstract

High-Performance Thin Layer Chromatography (HPTLC) is an advanced form of thin-layer chromatography that offers improved resolution, sensitivity, and reproducibility. It has emerged as a powerful analytical technique widely used in pharmaceutical, chemical, environmental, and food analysis. HPTLC enables the simultaneous analysis of multiple samples with minimal solvent consumption, making it cost-effective and environmentally friendly. This article provides a comprehensive overview of HPTLC, including its principles, instrumentation, methodology, validation parameters, and applications. The role of HPTLC in the standardization of herbal medicines and quality control of pharmaceuticals is highlighted. Recent advancements such as hyphenated techniques and digital imaging systems are also discussed. Despite some limitations, HPTLC remains a versatile and reliable tool in modern analytical science, offering significant advantages over conventional chromatographic techniques.

Anil Kumar Sharma

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