All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article Open Access

In-Vitro Inhibitory Effect Of Different Extracts of Chaetomium Cupreum on Alpha Glucosidase, Alpha Amylase and Hepg2 Cancer Cells


Objective: The present study investigates the effect of soil isolated fungus C. cupreum on alpha glucosidase, alpha amylase and HepG2 cancer cells.

Methods: The antidiabetic effect of C. cupreum extracts was determined by α-glucosidase and α-amylase inhibition assays and anticancer activity was done by MTT assay.

Results: The inhibitory effect on α-glucosidase enzyme by C. cupreum extracts was observed in ethyl acetate extract with the value of 57.88 ± 0.05, followed by n-butanol extract 21.27 ± 0.04, methanol extract 15.19 ± 0.05 and chloroform extract 12.34 ± 0.01 at 100 μg/ml. Whereas, the inhibitory effect on α-amylase enzyme by n-butanol extract with a value of 53.70 ± 0.10, followed by ethyl acetate extract 31.81 ± 0.05, methanol extract 20.51 ± 0.05 and chloroform extract 16.00 ± 0.05 at 100 μg/ ml concentration. In anticancer activity, n-butanol extract of C. cupreum showed significant cytotoxic effect against HepG2 cancer cells with IC50 value at 300 μg/ml.

Conclusion: Our study concludes that C. cupreum extracts possess potential phytochemicals that will be useful in antidiabetic and anticancer treatment after their purification and characterization

Nazir Ahmad Wani, Sharmila Tirumale*

To read the full article Download Full Article | Visit Full Article