Research Article Open Access

Simultaneous Estimation of Linagliptin and Metformin HCL by Simple Precise UV Spectroscopic Method in Bulk and Marketed Tablets

Abstract

The present work was aimed to develop and validate spectrophotometric methods for simultaneous estimation of Linagliptin (LIN) and Metformin HCl (MeT) in a pure and tablet dosage form. The absorption maxima were measured for LIN and MeT in solvent blend Methanol: Distilled water at 1:1 was 293 nm and 236 nm respectively. This method was validated according to ICH guidelines. The percentage estimation of LIN (Linazex 5, Linares 5), MeT (Vrimet 500, Vrimet 250) and percentage estimation of LIN and MeT in combined marketed tablet (Linaplizin Duo) was done in proposed method. A linearity range for LIN and MeT is 5-30 μg/ml at respective wavelengths. The coefficient of correlation for LIN at 293 nm and for MeT at 236 nm is 0.9998 and 0.9999, respectively. The percentage recovery from the marketed tablets by method is 98.92 ± 0.481 to 100.01 ± 0.279 for LIN (Linazex-5, Linares-%), 99.58 ± 0.963 to 99.87 ± 0.531 for MeT (Vrimet-500, Vrimet-250) and 98.97 ± 0.207, 100.2 ± 0.531 for LIN and MeT in (Linaplizin Duo) with a % RSD less than 2. The proposed method was robust and can be successfully rugged and stable for 72 hr. The results conclude that the proposed method was simple, rapid, and validated and can be used successfully used for routine simultaneous estimation of LIN and MeT in a single and combined tablets.

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