Research and Reviews - International Journals

Research & Reviews: Journal of Material Sciences

Menu

ISSN:2321-6212

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Dual-faced nano-mushrooms for tri-functional single cell diagnosis and drug delivery


11th International Conference on Advanced materials & Processing

September 07-08, 2017 | Edinburgh, Scotland

Hsin-Yi Hsieh, Tsu-Wei Huang,Chau-Hwang Lee and Fan-Gang Tseng

National Tsing Hua Univerity, Taiwan
Applied Science Research Center and Academia Sinica, Taiwan

Posters & Accepted Abstracts: Res. Rev. J Mat. Sci.

DOI: 10.4172/2321-6212-C1-006

Abstract

Here we introduce a monodispersed mushroom-like fluorescent nanoparticle with dual-faces and tri-functions for SERS-active Raman sensing, fluorescence detecting, cancer marker targeting, and drug carrying and delivering inside a cell. A one-step oxygen plasma process was employed to tailor commercial-available fluorescent PS beads into corrugated hemispheres and simultaneously modify the entire surface with carboxylic groups, and then a gold film was coated on the corrugated hemisphere for SEARs. Sulfo- NHS-SS-biotin disulfide linker and anti-CD44 monoclonal antibody could be modified simultaneously onto the top gold surfaces and bottom carboxyl groups through Au-S and peptide bonds, respectively. In exploiting the dual-module surface, highly selective modifications were performed to the Au-S bond using thiols and/or the peptide bond using a dehydration reaction between –COOH and –NH2. For applications in cancer, the DFPSBs were modified by attaching anti-CD44 antibody (on the carboxylated polystyrene) and a sulfo-NHS-SS-biotin disulfide linker (onto the amine or gold surface). The anti-CD44-modified DFPSBs can be utilized to target cancer cells (such as HeLa and MCF-7) with CD44 over-expressed. For drug delivery, a relatively weak covalent bond in the disulfide linker has the advantage of being capable of cleavage via reduction. The disulfide cleavage, dividing one R-SS-R into two R-SH molecules, occurs with cell cytoplasm environment. In the intracellular space, the cell regulatory mechanism can retain the redox equilibrium; consequently, the disulfide linker-modified DFPSBs act as vehicles releasing their load inside the cell membrane. Therefore, surface-modified DFPSBs can integrate three functions on the nanoparticles for biomedical applications.