Karolína M. Siskova
Palacký University in Olomouc, Czech Republic
ScientificTracks Abstracts: Res. Rev. J Mat. Sci.
Nanoparticles (NPs) are currently the topic of interest of a bundle of research papers and proposals. There are persistent rumors about NPs toxicity and ecotoxicity, concerns about their increasing release into the environment and attempts to their risk assessment. Many researchers often test the impact of different kinds of NPs on cells, tissues, organs, and/or directly on whole organisms. However, in order to understand the possible NPs impacts it is necessary, in my opinion, at first to evaluate the interaction of NPs with one type of the basic units of all organisms and their cells, i.e. amino acids. Furthermore, it is important to elucidate whether and how can NPs affect the conformation of proteins (their secondary and tertiary structure). Only after gaining such information, the assessment of NPs toxicity and ecotoxicity could be meaningful. In this study, the interaction of synthesized and commercially available nanoparticles with selected amino acids and a physiologically important transmembrane protein, the sodium/ potassium pump (Na+/K+-ATPase) will be investigated by using several spectroscopic techniques. The sodium/potassium pump is an enzyme which is ubiquitous in all animal cells and its malfunctions are related to many diseases such as hypertension, diabetic neuropathies, renal failure, neurological disorders etc.
Karolína M. Šišková has her expertise in nanoparticle synthesis, characterization, application and in vibrational spectroscopy. She published more than 40 papers as the main author or co-author in impacted scientific journals and presented more than 20 oral contributions and 8 posters on different nanomaterial and spectroscopic conferences and meetings. She graduated in physical chemistry from the Charles University in Prague and in INTER///BIO from the Université Pierre et Marie Curie, Paris VI, in 2006. In 2014, she was awarded the conferment of associate professor degree in physical chemistry. Her h-index is 15 and the number of citations 515 (without self-citations) according to the Web of Science on 20th December 2016. Coming back from her maternity leave in September 2016, she started to work at the Department of Biophysics, Faculty of Science, Palacký University in Olomouc, Czech Republic.