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RRJOMS | Volume 5 | Issue 4 | July, 2017
July 27-29, 2017 Vancouver, Canada
10
th
International Conference on
Emerging Materials and Nanotechnology
Hydroxyapatite–heparin–BMP-2 on modified titanium surfaces enhances the efficacy of bone formation
and osseointegration:
In vitro
&
in vivo
study
Deok-Won Lee
1
and
Sung ok Hong
2
1
Kyung Hee University, South Korea
2
Catholic Kwandong University, South Korea
Introduction:
In the present study, surface-modified Ti samples with hydroxyapatite (HAp) and heparin (Hep) bone morphogenetic
protein-2 (BMP-2) complex (Ti/HAp/Hep/BMP-2) were prepared and the effects of the samples on the enhancement of bone
formation and osseointegration in-vitro and in-vivo were investigated, as compared to Ti/HAp and Ti/Hep/BMP-2.
Methods & Materials:
Surface-modified titanium (Ti) samples with hydroxyapatite (HAp) and heparin (Hep)–bone morphogenetic
protein-2 (BMP-2) complex (Ti/HAp/Hep/BMP-2) were prepared, and their efficacies on the enhancements of bone formation and
osseointegration in-vitro and in-vivo were examined, and then compared to Ti/HAp and Ti/Hep/BMP-2. The modified surfaces were
characterized by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and contact angle goniometry.
Results:
In-vitro studies revealed that MG-63 human osteosarcoma cell lines grown on Ti/HAp/Hep/BMP-2 increased the amounts
of alkaline phosphatase (ALP) activity, calcium deposition and the levels of OCN mRNA gene expression as compared to those
grown on Ti/HAp, Ti/Hep/BMP-2 or pristine Ti. Moreover, Ti/HAp/Hep/BMP-2 exhibited higher bone volume (BV), bone volume/
tissue volume (BV/TV), removal torque value and bone–implant contact (BIC) than Ti/HAp, Ti/Hep/BMP-2 or pristine Ti
in vivo
.
Histological evaluations showed that many desirable features of bone remodeling existed at the interface between Ti/HAp/Hep/BMP-
2 and the host bone.
Conclusion:
Consequently, Ti/HAp/Hep/BMP-2 may have potential for clinical use as dental or orthopedic implants.
This study was supported by a grant from the National Research Foundation of Korea (NRF-2014R1A1A1002630 and NRF-
2016R1A2B4014600)
Biography
Deok-Won Lee is an Oral and Maxillofacial Surgery Specialist and Associate Professor of Kyung Hee University College of Dentistry. His expertise is in treating
and improving the oral and maxillofacial health and wellbeing of people. His research on dental implant materials creates new pathways for improving healthcare.
He is continually building and investigating on adequate material for implantation through
in-vivo
and
in-vitro
models based on years of experience in research,
evaluation, teaching and administration both in hospital and education institutions..
verycutebear@hanmail.netDeok-Won Lee et al., Res. Rev. J Mat. Sci. 2017
DOI: 10.4172/2321-6212-C1-002